Preparation method of genetic marker for high intramuscular fat (IMF) content in breast muscle of Hetian black chicken and application of genetic marker

Abstract

The invention discloses a preparation method of a genetic marker for high intramuscular fat (IMF) content in breast muscle of a Hetian black chicken and an application of the genetic marker. The method comprises the following steps: selecting 60 120-day-old Hetian black chicken, which include 30 hens and 30 cocks; and detecting the second exon polymorphism of H-FABP (heart fatty acid-binding protein) and the IMF content in the breast muscle, and analyzing the relationship between the second exon polymorphism and the content. An experiment shows that the Hetian black chicken with high IMF content in breast muscle is selected by the genetic marker prepared by the method.

Description

technical field [0001] The invention belongs to the technical field of poultry genetic markers, and relates to a preparation method and application of a genetic marker for high intramuscular fat content in Hetian black chicken breast muscle. Background technique [0002] With the improvement of people's living standards, there are stricter requirements on the quality and taste of meat. Chicken is the main meat on the table, and the quality of chicken has increasingly become the focus of attention. Most of the standards for measuring the quality of chicken meat come from people's taste, and there is no unified standard at present. After analysis, the high intramuscular fat IMF in chicken is considered to be a main indicator affecting the meat quality traits of chicken and improving the tenderness of meat. When the high intramuscular fat content reaches more than 3.5%, good meat flavor can be obtained. [0003] With the development of molecular biology, people try to use gene...

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Most of the standards for measuring the quality of chicken meat co

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