Genetic bar code detection kit of dermestidae insect

A detection kit and barcode technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of insufficient system reports, achieve high amplification efficiency, simple operation, and reduce the quality of specimens. Effect

Inactive Publication Date: 2015-08-19
郑斯竹
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Problems solved by technology

However, there are still not many related systematic reports at home and abroad on the rapid detection technology of gene barcodes for dermatomids

Method used

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  • Genetic bar code detection kit of dermestidae insect

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Effect test

Embodiment 1

[0028] A gene barcode detection kit for Dermestidae insects, which can quickly detect 19 kinds of dermestidae (as shown in Table 1), including at least 20 times of primer J1837 / N2110 reagent and 1 gene barcode sequence disc; primer J1837 / N2110 The N2110 sequence is as follows:

[0029] J1837 sequence: 5'-TATAGCATTCCCACGAATAAATA-3',

[0030] N2110 sequence: 5'-ATTTCGGTCAGTTAATAATATAG-3'.

[0031] The gene barcode sequence disk contains 19 kinds of 61 standard barcode sequences, and the detailed sequences are shown in SEQ ID NO.1-61.

[0032] Table 1 Dermatomid species name

[0033] serial number Chinese name Latin name 1 Small round beetle Anthrenus verbasci 2 Specimen orb beetle Anthrenus museorum 3 Carpet beetle Anthrenus scrophulariae 4 two-star fur beetle Attagenus pellio 5 Derma beetle Attagenus smirnovi 6 A species of fur beetle Attagenus woodroffei

[0034] 7 black fur beetle Attagenus unic...

Embodiment 2

[0037] Seven samples of Trogoderma zebra, Trogoderma cerevisiae, Dermoderma cerevisiae, Dermoderma spp., Beetle grandis, Bean beetle four-striped and Rice-beetle were selected as the samples to be tested. Adopt the kit detection of embodiment 1, the steps are as follows:

[0038] 1) Total DNA extraction

[0039] DNA was extracted using GenMagBio Animal Cell Tissue / Cell Genomic DNA Magnetic Beads Extraction Kit. The specific process is as follows:

[0040] Directly cut 30 mg of the feet and chest of the test specimen and put it into a 2 mm test tube. Soak the preserved specimen in absolute ethanol, rinse and soak it with sterile distilled water for 4 to 5 times, and discard the water; soak the dry specimen in sterile distilled water for 3 hours and blot dry. Place in a 2mL centrifuge tube, shake and grind in MM400 ball mill (30 times / s) for 30s, add 180μL Lysis Buffer, 20μL Proteinase K, shake and mix well, leave at room temperature overnight, shake and warm at 55°C for 3-5h,...

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Abstract

The invention discloses a genetic bar code detection kit of a dermestidae insect. The kit can be used for quickly detecting 19 carpet beetles, comprises at least one primer J1837/N2110 of more than 20 doses and a genetic bar code sequence disc, wherein the primer J1837 sequence is 5'-TATAGCATTCCCACGAATAAATA-3', and the primer N2110 sequence is 5'-ATTTCGGTCAGTTAATAATATAG-3'; 61 standard code sequences are stored in the genetic bar code sequence disc; the detailed sequences are shown from SEQ ID NO.1 to SEQ ID NO. 61. The genetic bar code detection kit of the dermestidae insect is an efficient, accurate and convenient dermestidae insect molecular detection technology; the types of 19 dermestidae insects can be distinguished in the molecular level; the use requirements can be met.

Description

technical field [0001] The invention relates to the technical field of dermestidae insect detection, in particular to a dermestidae insect gene barcode detection kit. Background technique [0002] Dermestidae insects are widely distributed all over the world and mainly attack stored animal and plant materials. Their populations have a variety of food habits and strong fecundity. They can endure extremely low humidity in the environment to complete life generations, resulting in the same quantity, quality and market value of stored commodities. Great loss and destruction. At present, the research on species identification of Dermestidae is still mainly focused on morphological identification. The traditional insect morphological identification method has certain limitations. The complete adult must be used as the morphological research object. The related species, early (egg, larva and pupa) Both the structure and the incomplete insect body are difficult to identify. With t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/16
Inventor 郑斯竹李枷霖詹国辉高渊王建斌陈云芳樊新华
Owner 郑斯竹
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