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Detection kit for human leucocyte antigen genes

A technology of leukocyte antigen and detection kit, which is applied in the direction of biological testing, microbial determination/testing, material inspection products, etc.

Pending Publication Date: 2015-08-26
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV +1
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Problems solved by technology

[0005] After searching the existing technical literature, it is found that there is no correlation between the HLA-B*59:01 gene and the pathogenesis of SJS and TEN caused by methazolamide in the Han population in mainland China, and it can be used as a genetic marker to screen for this type. Reports about drug rash

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  • Detection kit for human leucocyte antigen genes
  • Detection kit for human leucocyte antigen genes
  • Detection kit for human leucocyte antigen genes

Examples

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Embodiment 1

[0112] 1) Collect and extract genes

[0113] Patients with SJS and TEN caused by methazolamide were from Huashan Hospital Affiliated to Fudan University, Shanghai, China;

[0114] The diagnostic criteria were proposed by Roujeau et al. (Roujeau JC. Clinical heterogeneity of drug hypersensitivity. Toxicology 2005; 209: 123-129.), that is, clinical manifestations include extensive exfoliation and necrosis of the epidermis, oral mucosal erosion, with or without severe liver and kidney disease Systemic complications such as functional impairment, among them, the area 30% is called TEN; 10% to 30% is called SJS / TEN overlap; those who participated in this project under the premise of informed consent Research, blood collection, and signed informed consent; all enrolled patients were excluded from other diagnoses such as viral eruption and toxic erythema through medical history and skin pathological examination, and all patients took oral methazolamide for the first time within 2 mon...

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Abstract

The invention belongs to the technical field of biological medicines and relates to a detection kit for human leucocyte antigen (HLA) genes--HLA-B*59: 01 genes, and the HLA-B*59: 01 genes can serve as marker genes for forecasting methazolamide caused SJS (Stevens-Johnson syndrome) and TEN (Toxic epidermal necrolysis). According to the detection kit, after DNA extracted from the peripheral blood of a patient, the PCR-SSO method is adopted to detect the HLA-B*59 :01 genes. The detection kit for HLA-B*59: 01 genes can be used for examining before application of methazolamide and screening targeted medicines for treating methazolamide caused SJS and TEN; and examination can guide clinical medication to reduce incidence of SJS and TEN caused by methazolamide, and the screening mainly acts on HLA-B*59: 01 molecules in a targeted manner, so that interaction between the HLA-B*59: 01 molecules and methazolamide or other metabolic products during attack.

Description

technical field [0001] The invention belongs to the field of biomedicine and reagent detection, and relates to a test kit for detecting human leukocyte antigen gene; Two types of cutaneous adverse drug reactions (Stevens-Johnson syndrome and toxic epidermal necrolysis) were associated. Background technique [0002] Drug eruption refers to the inflammatory reaction of skin and mucous membranes caused by drugs entering the human body through various channels. Stevens-Johnson syndrome (Stevens-Johnson syndrome, SJS) and toxic epidermal necrolysis (Toxic epidermal necrolysis, TEN) belong to the same spectrum of severe cutaneous adverse drug reactions. exfoliation and necrosis of severe hepatic and renal dysfunction (Bastuji-Garin S, Rzany B, Stern RS, Shear NH, Naldi L and Roujeau JC. Clinical classification of cases of toxic epidermal necrolysis, Stevens- Johnson syndrome, and erythema multiforme. Arch Dermatol 1993;129:92-96.). According to statistics, the annual incidence ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/68
Inventor 骆肖群邢清和贺林杨凡萍陈圣安
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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