Manufacturing method of sutures used for MACO models of mice

Abstract

Provided is a manufacturing method of sutures used for MACO models of mice. The sutures are prepared by selection, preparation of material, coating of head ends and marking after coating. The suture material is a filament which is drawn with the diameter ranging from 0.065 to 0. 1 mm at the temperature of 300 DEG C and made by polytetrafluoroethylene resin. At the temperature of 350 DEG C, the surface of the above filament is coated by titanium dioxide in order to obtain the material of the sutures. Coating material, for coating the head ends, is formed by mixing 15% of silicone emulsion, 34% of polyvinylpyrrolidone, 23% of polyvinyl alcohol, 16% of polyethylene glycol, and 12% of polytetrafluoroethylene under ultrasonic concussion based on weight. The manufacturing method of the sutures used for MACO models of mice has following beneficial effects: the sutures are consistent in size and have appropriate hardness and elasticity and blunt and smooth head ends so that the sutures can smoothly enter middle cerebral arteries of mice; inserted sutures can be bent in accordance with the bending blood vessels in order to reduce mechanical damage to blood vessels; obstruction is performed more completely and reliably; the scope of cerebral ischemia is stable; cerebral infarct volume is stable; operation is carried out easily; high success rate of models is achieved; and death rate of mice is low.

Description

technical field [0001] The invention belongs to the field of life sciences, and in particular relates to a method for making a thread plug for a mouse MCAO model. Background technique [0002] Stroke is a general term for acute cerebrovascular diseases, clinically manifested as hemiplegia, aphasia and other symptoms, and has the characteristics of high fatality rate, high disability rate and high recurrence rate. The latest research shows that stroke has seriously threatened people's life and quality of life, among which ischemic stroke ranks first among patients, accounting for about 80% of all stroke patients. [0003] The animal model of ischemic stroke is the basis for the study of cerebral ischemic injury and treatment. The middle cerebral artery is a predilection site for ischemic stroke. Therefore, the middle cerebral artery occlusion (MCAO) animal model is often used as the research basis. The embolization middle cerebral artery cerebral ischemia model is the prefer...

AI Technical Summary

Problems solved by technology

Commonly used glue coating methods include paraffin-coated, silica-gel-coated, polyhexenol thread, etc. Among them, the paraffin-coated thread is easy to fall off; although the infarct area is relatively stable with the silicone-coated thread plug, it has no expansion effect, making the infarction incomplete , and the mortality rate is high, and it is not easy to observe and study for a long time; the polyhexenol thread plug is not hard enough and will swell when encountering blood, making the thread entry difficult