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Au@ p-mercapto-phenylmercaptan @Au combination Raman probe and production method thereof

A technology of p-mercaptobenzenethiol and Raman probes, applied in the field of bio-nano, can solve the problems of easy loss or denaturation of Raman molecules, sensitivity and accuracy, etc., and achieve the ability of easy loss or denaturation, environmental impact resistance Strong, reproducible results

Inactive Publication Date: 2015-09-09
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, Raman molecules are directly modified on the surface of gold nanoparticles, and Raman molecules are easily lost or denatured during centrifugation, washing and other operations in subsequent experiments, which affects the sensitivity and accuracy of detection.

Method used

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  • Au@ p-mercapto-phenylmercaptan @Au combination Raman probe and production method thereof
  • Au@ p-mercapto-phenylmercaptan @Au combination Raman probe and production method thereof
  • Au@ p-mercapto-phenylmercaptan @Au combination Raman probe and production method thereof

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Embodiment 1

[0037] Preparation of nuclear gold nanoparticles.

[0038] The steps are as follows: add 150mL 2.2mM Na in a 250mL three-neck flask 3 C 6 h 5 o 7 , while stirring (1500r·min -1 ), heated to boiling under the action of condensing reflux, added 1mL 25mM HAuCl 4 , after 10min the color of the solution turned red and became stable. After 30min, the temperature was lowered to 90°C (denoted as g0), and 1mL of 25mM HAuCl was added 4 , react for 30min; add 1mL 25mM HAuCl again 4 , After reacting for 30min, stir and cool to room temperature (denoted as g1).

[0039] Take 55mL of the above solution, add 2mL of 60mM Na 3 C 6 h 5 o 7 , add 53mL of ultrapure water, raise the temperature to 90°C, and repeat the operation of g1 once. That is, a solution containing gold nanoparticles (denoted as g2) is obtained. Morphology of nuclear gold nanoparticles figure 2 as shown, figure 2 It can be seen that the nanoparticles are uniformly spherical in shape.

[0040] Preparation of A...

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Abstract

The invention discloses an Au@ p-mercapto-phenylmercaptan @Au combination Raman probe and a production method thereof. The probe is composed of internal core gold nanoparticles, a Raman molecule interlayer and an outer gold shell layer. The production method includes adding chloroauric acid prepared core nanogold into sodium citrate solution, fitting p-mercapto-phenylmercaptan to the core nanogold, and preparing the gold shell layer on the surface of the p-mercapto-phenylmercaptan to obtain the Au@ p-mercapto-phenylmercaptan @Au combination Raman probe. In the probe, Raman signal molecules, the p-mercapto-phenylmercaptan, are located in the interlayer between the core nanogold and the gold shell layer, insusceptible to the ambient environment and are of good stability; the gold surface of the probe has uniform chemical property, the probe is suitable for modifying various biological molecules and thus is applicable to the detection of various biological markers and widely applicable.

Description

technical field [0001] The invention relates to a preparation method of an Au p-mercaptobenzenethiol Au composite Raman probe, belonging to the field of biological nanometers. Background technique [0002] Cancer has become one of the major diseases threatening human health in the 21st century. Therefore, accurate detection of the content of cancer markers has become an urgent problem to be solved. The application of gold nanoparticles in Raman detection can amplify the Raman signal and realize the sensitive detection of cancer markers. However, if Raman molecules are directly modified on the surface of gold nanoparticles, Raman molecules are easily lost or denatured during centrifugation, washing and other operations in subsequent experiments, which affects the sensitivity and accuracy of detection. Therefore, solving the defects of the existing nano-gold modified Raman molecules is the key technology for its further development. Contents of the invention [0003] Aimi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 向娟郑荣荣
Owner CENT SOUTH UNIV
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