Method of producing microarray aptamer sensor used for antibiotic residue detection

An aptamer sensor and tetracycline aptamer technology, applied in the direction of material resistance, can solve problems such as difficult cleaning, thin gold layer, and easy damage, and achieve low cost, strong anti-interference ability, and simple and easy operation Effect

Inactive Publication Date: 2015-09-23
SHANDONG UNIV OF TECH
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Problems solved by technology

The interdigitated array microelectrode (IDAM) has the characteristics of low impedance drop, can quickly establish a steady-state signal, and improve the signal-to-noise ratio. However, due to the uneven surface of the electrode and the thin gold layer, it is not easy to clean and easily damaged. Very limited; the purpose of the present invention is to provide a method for preparing an aptamer sensor that can overcome the above-mentioned defects and is simple in operation, high in sensitivity and good in selectivity to detect tetracycline residues

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  • Method of producing microarray aptamer sensor used for antibiotic residue detection
  • Method of producing microarray aptamer sensor used for antibiotic residue detection
  • Method of producing microarray aptamer sensor used for antibiotic residue detection

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Embodiment Construction

[0030] The present invention will be further described below by the examples, but the present invention is not limited by the examples.

[0031] Electrode cleaning method is as follows:

[0032] The gold interdigitated microarray electrodes used in this example were purchased from AB-tech Scientific Company of the United States. The electrodes consisted of 50 pairs of interdigitated array electrodes with a finger width of 15 μm and a finger spacing of 15 μm. Electrode cleaning method is as follows:

[0033] Immerse the gold finger microarray electrode in 1M sodium hydroxide solution for 15 minutes, wipe the surface of the electrode with lens cleaning paper for 2 minutes, rinse it with ultrapure water, then immerse the electrode in 1M hydrochloric acid solution, soak for 15 minutes, wipe the electrode surface with After wiping the surface of the electrode with paper for 2 minutes, rinse it with ultrapure water and blow it dry with nitrogen; observe the cleaned electrod...

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Abstract

The invention relates to a method of producing a microarray aptamer sensor used for antibiotic residue detection. A microarray electrode aptamer sensor has the advantages of being higher in sensitivity and wider in linear response range. The microarray aptamer sensor provided by the invention is used for quick detection of tetracycline residues in milk, and the detection includes the following steps: firstly cleaning a microarray electrode with alkaline and acid alternately, modifying the microarray electrode using ionic liquid and ferroferric oxide, fixing a tetracycline aptamer on the modified microarray electrode to obtain the interdigital microarray aptamer sensor, and then carrying out in-situ quick detection of the tetracycline residues in milk. The sensor provided by the invention is high in sensitivity, stability and specificity, and good in regeneration capability; the recovery rate meets requirements; therefore, the sensor is quite suitable for occasions of antibiotic residue in-situ quick detection.

Description

technical field [0001] The invention relates to a method for preparing a microarray aptamer sensor for antibiotic residue detection, and belongs to the technical field of biosensor preparation. Background technique [0002] In recent years, with the rapid development of my country's animal husbandry industry, milk production has increased significantly, and fresh milk and dairy products have become an important part of people's (especially the elderly and children's) daily food. Antibiotics are widely used to prevent and treat dairy cow diseases. Due to frequent and overdose use, there are a certain amount of antibiotic residues in milk and other animal source foods. Drinking or eating milk or dairy products with antibiotic residues for a long time is equivalent to long-term low-dose antibiotic intake, which will inhibit the normal flora in the human intestinal tract and cause a large number of pathogenic bacteria to cause systemic or local infection. Due to the high incide...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/06
Inventor 郭业民孙霞胥清翠王相友
Owner SHANDONG UNIV OF TECH
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