Application of salicylic acid glycolipid derivatives in accumulating intracellular alkaloids of periwinkle callus
A salicylic acid glycolipid, callus technology, applied in the application field of salicylic acid glycolipid derivatives, can solve problems such as unsatisfactory effect and the like
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[0020] Compound (I-1)-compound (I-4) were respectively dissolved in deionized water to prepare 1 ppm and 10 ppm aqueous solutions.
[0021] The periwinkle callus cells are placed in a 250mL Erlenmeyer flask containing 50mL of culture solution, and the inoculation amount is 1.5-2g / bottle. The culture solution is MS culture solution containing compound (I-1)-compound (I-4) each 1mg / L (in the form of aqueous solution), 3wt% sucrose, pH6.0 (before sterilization), at 25°C, Shaker (100r·min -1 ), cultured under light for 12h. The cultured periwinkle callus cells (that is, the culture after filtering the culture medium) were homogenized with 80% methanol aqueous solution, extracted overnight, and the methanol was evaporated under reduced pressure, and the pH value of the water phase was adjusted to 3. Then extract with petroleum ether, adjust the pH value of the water phase to 8.5, extract with dichloromethane, measure the OD value of the organic layer at 280mm, and evaporate the d...
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