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Application of salicylic acid glycolipid derivatives in accumulating intracellular alkaloids of periwinkle callus

A salicylic acid glycolipid, callus technology, applied in the application field of salicylic acid glycolipid derivatives, can solve problems such as unsatisfactory effect and the like

Active Publication Date: 2018-05-08
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, people have tried to increase the content of indole alkaloids by using tissue and cell culture methods, combined with optimized culture conditions, precursor feeding or treatment with various regulatory factors, but the results are not satisfactory

Method used

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  • Application of salicylic acid glycolipid derivatives in accumulating intracellular alkaloids of periwinkle callus
  • Application of salicylic acid glycolipid derivatives in accumulating intracellular alkaloids of periwinkle callus
  • Application of salicylic acid glycolipid derivatives in accumulating intracellular alkaloids of periwinkle callus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Compound (I-1)-compound (I-4) were respectively dissolved in deionized water to prepare 1 ppm and 10 ppm aqueous solutions.

[0021] The periwinkle callus cells are placed in a 250mL Erlenmeyer flask containing 50mL of culture solution, and the inoculation amount is 1.5-2g / bottle. The culture solution is MS culture solution containing compound (I-1)-compound (I-4) each 1mg / L (in the form of aqueous solution), 3wt% sucrose, pH6.0 (before sterilization), at 25°C, Shaker (100r·min -1 ), cultured under light for 12h. The cultured periwinkle callus cells (that is, the culture after filtering the culture medium) were homogenized with 80% methanol aqueous solution, extracted overnight, and the methanol was evaporated under reduced pressure, and the pH value of the water phase was adjusted to 3. Then extract with petroleum ether, adjust the pH value of the water phase to 8.5, extract with dichloromethane, measure the OD value of the organic layer at 280mm, and evaporate the d...

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Abstract

The invention discloses the application of a salicylic acid glycolipid derivative in accumulating alkaloids in periwinkle callus cells, and the alkaloids are total indole alkaloids, vinblastine or amarine; The salicylic acid glycolipid compound has a cumulative effect on total indole alkaloids, vinblastine or amarine in periwinkle callus cells, and the compound provides a basis for the development of new drugs.

Description

(1) Technical field [0001] The invention relates to the application of a salicylic acid glycolipid derivative, in particular to the application of the salicylic acid glycolipid derivative in accumulating alkaloids in periwinkle callus cells. (2) Background technology [0002] Periwinkle is native to East Africa, and is currently grown in many areas of my country as medicinal plants and ornamental plants. The main medicinal components of periwinkle are indole alkaloids. Among them, vinblastine (vinblastine) and vincristine (vincfistine) are the most widely used natural plant anti-tumor drugs at present, and can be used to treat Hodgkin's disease, malignant lymphoma, acute leukemia, villous epithelial cell carcinoma and other diseases. Salt has been widely used clinically; in addition, ajmalicine (ajmalicine) can be used to treat diseases such as hypertension, arrhythmia; Serpentine (serpentine) can be used as analgesic; , diuretic and hypoglycemic effects, but also the synt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/12C12P1/00
Inventor 刘幸海杨明艳杨二冰臧洪俊王勇李正名
Owner ZHEJIANG UNIV OF TECH
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