Method for extracting and separating vinblastine from periwinkle by enzyme-assisted complex extraction

A technology for vinblastine and periwinkle, which is applied in the field of efficient extraction of vinblastine, can solve problems such as hindering the extraction rate of active components and difficult release of target products, and achieves the effects of improving resource utilization, easy industrialization and convenient operation.

Active Publication Date: 2017-09-15
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] During the extraction of plant active ingredients, due to the thicker cell walls outside the plant cells, the target products inside the cells are not easily released into the solvent, which hinders the extraction rate of active ingredients

Method used

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  • Method for extracting and separating vinblastine from periwinkle by enzyme-assisted complex extraction
  • Method for extracting and separating vinblastine from periwinkle by enzyme-assisted complex extraction
  • Method for extracting and separating vinblastine from periwinkle by enzyme-assisted complex extraction

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Experimental program
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Effect test

Embodiment 1

[0041] The method for extracting and separating vinblastine from periwinkle by enzyme-assisted complex extraction method comprises the following steps:

[0042] (1) Enzyme-assisted ultrasonic extraction

[0043] Take 100g of periwinkle aboveground part and dry it in the shade and crush it into powder to get periwinkle dry powder, add 400ml of mixed enzyme solution to hatch, the temperature of incubation is 25°C, and the time is 6h; after the hatching, filter, keep periwinkle powder, add periwinkle powder 5 times the weight of 70% ethanol, adjust the pH to 5.6 with hydrochloric acid, ultrasonically extract 3 times, each time for 6 hours; filter, keep the extract, and concentrate the extract under reduced pressure to obtain a concentrate;

[0044] The mixed enzyme solution includes: cellulase 3U / ml, pectinase 4U / ml, hemicellulase 6U / ml; the pH of the mixed enzyme solution is 4.0, and the mixed enzyme solution also includes metal ion Ca 2+ , Ca 2+ The mass fraction is 1%;

[0...

Embodiment 2

[0054] The method for extracting and separating vinblastine from periwinkle by enzyme-assisted complex extraction method comprises the following steps:

[0055] (1) Enzyme-assisted ultrasonic extraction

[0056] Take 100g of periwinkle aboveground part and dry it in the shade and crush it into powder to get periwinkle dry powder, add 400ml of mixed enzyme solution to hatch, the temperature of incubation is 30°C, and the time is 6h; after the hatching, filter, keep periwinkle powder, add periwinkle powder 5 times the weight of 70% ethanol by volume, and adjusting the pH to 5.8 with hydrochloric acid, ultrasonically extracting 4 times, 5 hours each time; filtering, leaving the extract, concentrating the extract under reduced pressure to obtain a concentrate;

[0057] The mixed enzyme solution includes: cellulase 4U / ml, pectinase 5U / ml, hemicellulase 7U / ml; the pH of the mixed enzyme solution is 5.0;

[0058] The vacuum degree of decompression concentration is -0.9MPa, and the r...

Embodiment 3

[0067] The method for extracting and separating vinblastine from periwinkle by enzyme-assisted complex extraction method comprises the following steps:

[0068] (1) Enzyme-assisted ultrasonic extraction

[0069] Take 100g of periwinkle aboveground part and dry it in the shade and crush it into powder to get periwinkle dry powder, add 400ml of mixed enzyme solution to hatch, the temperature of incubation is 40°C, and the incubation time is 5h; after hatching, filter, keep periwinkle powder, add periwinkle powder 5 times the weight of 80% ethanol, adjust the pH to 6.2 with hydrochloric acid, ultrasonically extract 3 times, each time for 5 hours; filter, keep the extract, and concentrate the extract under reduced pressure to obtain a concentrate;

[0070] The mixed enzyme solution includes: cellulase 5U / ml, pectinase 8U / ml, hemicellulase 9U / ml; the pH of the mixed enzyme solution is 6.0, and the mixed enzyme solution also includes metal ions Mn 2+ , Mn 2+ The mass fraction is 1...

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Abstract

The invention discloses a method for extracting and separating catharanthine from Catharanthus roseus by using an enzyme assisted complexometric extraction technology. The method comprises the following steps: 1, carrying out enzyme assisted ultrasonic extraction on catharanthine from Catharanthus roseus, and carrying out reduced pressure concentration on the above obtained extract liquid; 2, carrying out complexometric extraction on the obtained concentrate by using an extraction system formed by D2EHPA used as a complexing agent and petroleum ether used as a diluent to obtain a catharanthine-rich extract liquid; 3, carrying out back extraction by using dilute hydrochloric acid to obtain crude catharanthine; and 4, purifying the crude catharanthine. The catharanthine including an amido group is alkaline, and undergoes complexometric extraction by using the acidic complexing agent D2EHPA to make the catharanthine react with the complexing agent in order to form a complex, the complex is transferred to an extraction phase, and back extraction is carried out with dilute hydrochloric acid to recovery the catharanthine. The extraction method effectively improves the recovery rate of the catharanthine, and has no pollution to environment due to recycling of the extractant used in the complexometric extraction.

Description

technical field [0001] The invention relates to a method for efficiently extracting vinblastine from periwinkle flowers, in particular to a method for extracting and separating vinblastine from periwinkle flowers by an enzyme-assisted complexation extraction method. Background technique [0002] Periwinkle (Catharanthus roseus) is an important medicinal plant of the genus Periwinkle in the family Apocynaceae, native to South Asia, East Africa and tropical America. In my country, it is mainly distributed in Jiangsu, Zhejiang, Fujian, Jiangxi, Sichuan and Henan. The whole plant of periwinkle can be used as medicine. It contains various terpene indole alkaloids such as vinblastine, vindolin, vinblastine, amaline, serpentine, vincristine, etc., and has high medicinal value. Among them, vinblastine not only has antitumor activity, but also has hypoglycemic, blood pressure, analgesic and other effects. At the same time, vinblastine is also an intermediate for the synthesis of vin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D471/22
CPCC07D471/22
Inventor 马科杨雪鹏钟桂芳叶建斌胡仙妹刘寅马歌丽邵化
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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