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A method for preparing fish skin collagen and ACE inhibitory peptide

A technology of fish skin collagen and inhibitory peptides, which is applied in the field of collagen extraction, can solve the problems of toxic substances, no bioavailability value, and tryptophan destruction, achieve fat-free, increase added value and resource utilization, and increase added value. value effect

Active Publication Date: 2016-05-25
吉林添正生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The acid method mostly uses strong acids such as hydrochloric acid and sulfuric acid to react at high temperature. The reaction is strong, the equipment is severely corroded, the hydrolysis is complete, and more amino acid mixtures are produced. However, tryptophan will be destroyed in this way, and it has been gradually eliminated. - Amino acids can also form toxic substances and have no bioavailability value, so they should not be used

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The method for preparing fish skin collagen and ACE inhibitory peptide is as follows:

[0026] (1) Remove impurities, wash the fish skin, freeze it at -15°C for 3 hours, and then put it into a planetary mill to grind it into a powder with a particle size of 200 microns;

[0027] (2) Use metallopeptidase Protease A to enzymolyze and inactivate fish skin powder. The enzymatic hydrolysis conditions are: fish skin powder concentration 8%, enzymolysis temperature 32°C, enzyme addition 0.4%, enzymolysis time 7h, enzyme The pH value of the solution was 7.5, the enzyme was inactivated at 95°C for 5 minutes, the enzymatic solution was filtered to obtain the supernatant and filter residue, the supernatant was concentrated and dried to obtain fish skin collagen, and the extraction rate was 57.48%;

[0028] (3) Using A.S.1398 neutral protease to enzymolyze and inactivate the collagen obtained in step (2), the enzymolysis conditions are as follows: the enzymolysis temperature is 55°...

Embodiment 2

[0030] The method for preparing fish skin collagen and ACE inhibitory peptide is as follows:

[0031] (1) Remove impurities, wash the fish skin, freeze it at -8°C for 5 hours, and then put it into a planetary mill to grind it into a powder with a particle size of 150 microns;

[0032] (2) Use metallopeptidase Protease A to enzymolyze and inactivate fish skin powder. The enzymatic hydrolysis conditions are: fish skin powder concentration 12%, enzymolysis temperature 28°C, enzyme addition 0.2%, enzymolysis time 3h, enzyme The pH value of the solution was 6.5, the enzyme was inactivated at 80°C for 15 minutes, the enzymatic solution was filtered to obtain the supernatant and filter residue, the supernatant was concentrated and dried to obtain fish skin collagen, and the extraction rate was 55.32%;

[0033] (3) Using A.S.1398 neutral protease to enzymolyze and inactivate the collagen obtained in step (2), the enzymolysis conditions are as follows: the enzymolysis temperature is 50...

Embodiment 3

[0035] The method for preparing fish skin collagen and ACE inhibitory peptide is as follows:

[0036] (1) Remove impurities, wash the fish skin, freeze it at -10°C for 4 hours, and then put it into a planetary mill to grind it into a powder with a particle size of 180 microns;

[0037](2) Use metallopeptidase Protease A to enzymolyze and inactivate fish skin powder. The enzymatic hydrolysis conditions are as follows: fish skin powder concentration 5%, enzymolysis temperature 30°C, enzyme addition 0.3%, enzymolysis time 5 hours, enzyme The pH value of the solution was 7, the enzyme was inactivated at 90°C for 10 minutes, the enzymatic solution was filtered to obtain the supernatant and filter residue, the supernatant was concentrated and dried to obtain fish skin collagen, and the extraction rate was 55.39%;

[0038] (3) Using A.S.1398 neutral protease to enzymolyze and inactivate the collagen obtained in step (2), the enzymolysis conditions are: enzymolysis temperature is 55°C...

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Abstract

The invention discloses a method for preparing fish skin collagen and ACE inhibitory peptide, which comprises the following steps of (1) carrying out impurity removal and cleaning on the fish skin, freezing at -15 to -8 DEG C for 3-5 hours, and then putting into a planetary mill to mill into powder in granularity of 150-200 micron; (2) carrying out enzymolysis and enzyme deactivation on the fish skin powder by using metallaopeptide Protease A, filtering the enzymatic hydrolysate to obtain supernate and filter residue, and concentrating and drying the supernate to obtain the fish skin collagen; and (3) carrying out enzymolysis and enzyme deactivation on the fish skin collagen obtained in the step (2), cooling and centrifuging to obtain supernate, carrying out vacuum concentration on the supernate, and finally carrying out freezing and drying. The preparation is wide in raw material source and simple in process, and has the effects of improving the extraction rate and the quality of the fish skin collagen and the ACE inhibitory peptide and improving the usage rate and the economic benefit.

Description

technical field [0001] The invention relates to the technical field of collagen extraction, in particular to a method for extracting collagen from fish skin. Background technique [0002] my country has vast waters, abundant aquatic product resources, and a large amount of aquatic product processing waste, which is rich in collagen content, but the degree of development and utilization of this in our country is relatively low. Collagen is an important structural protein of animal connective tissue and the most abundant protein in animals. It has good biocompatibility, biodegradability, and immunological properties. It is widely used in food industry and biomedical raw materials. Collagen peptides obtained from collagen hydrolysis have many physiologically active functions, such as ACE inhibitory peptides, anti-tumor peptides, and antioxidant peptides. The preparation of collagen and ACE inhibitory peptides from a large amount of aquatic product processing waste not only make...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K14/00A61P9/12A61P3/06
Inventor 唐亚丽卢立新雷亚君
Owner 吉林添正生物科技股份有限公司
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