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Synthetic culture medium capable of inducing catharanthus roseus cells to secrete catharanthine

A technology of synthetic medium and cell secretion, which is applied in the direction of cell culture medium, cell culture active agent, plant cells, etc., and can solve the problems of low vinblastine yield and slow cell growth, etc.

Inactive Publication Date: 2017-05-31
天津市博爱生物药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the technical defects of the prior art, and provides a synthetic medium for inducing vinca cells to secrete vinblastine, so as to solve the technical problem of slow cell growth in the prior art when vinca cells are cultured in vitro
[0005] Another technical problem to be solved by the present invention is that the productivity of vinblastine in the prior art is relatively low when the periwinkle cells are cultured in vitro

Method used

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  • Synthetic culture medium capable of inducing catharanthus roseus cells to secrete catharanthine

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Effect test

Embodiment 1

[0018] A synthetic medium for inducing vinca cells to secrete vinblastine. The medium contains the components of MS medium, and also contains phytohormone 2.4-D 2mg / L, phytohormone KT 1mg / L, tryptophan 50mg / L , coenzyme A 3mg / L, naproxen 8.4mg / L, aspirin 0.3mg / L, ibuprofen 5.0mg / L.

Embodiment 2

[0020] A synthetic medium for inducing vinca cells to secrete vinblastine. The medium contains the components of MS medium, and also contains phytohormone 2.4-D 2mg / L, phytohormone KT 1mg / L, tryptophan 50mg / L , Coenzyme A 3mg / L, Naproxen 8.4mg / L, Aspirin 0.3mg / L, Ibuprofen 5.0mg / L, Daidaihua squeezed juice 1g / L.

Embodiment 3

[0022] A synthetic medium for inducing vinca cells to secrete vinblastine. The medium contains the components of MS medium, and also contains phytohormone 2.4-D 2mg / L, phytohormone KT 1mg / L, tryptophan 50mg / L , coenzyme A 3mg / L, naproxen 8.4mg / L, aspirin 0.3mg / L, ibuprofen 5.0mg / L, breviscapine extract juice 1g / L.

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Abstract

The invention provides a synthetic culture medium capable of inducing catharanthus roseus cells to secrete catharanthine. The culture medium contains components of an MS culture medium, and also contains 2mg / L of plant hormone 2.4-D, 1mg / L of plant hormone KT, 50mg / L of tryptophan, 3mg / L of coenzyme A, 8.4mg / L of naproxen, 0.3mg / L of aspirin and 5.0mg / L of ibuprofen. On the basis, the culture effect is improved by adding a plant extraction manner; specifically, a physical squeezing manner is used for obtaining liquid components in fresh Chinese herbal medicines including bitter citrus immature flowers, herba erigeromtis, ocimum gratissimum, psammosilene tunicoides and the like, and the liquid components are directly added into the culture medium of the formula; a good culture effect is obtained, and the growth speed of cells and the final density are remarkably improved; meanwhile, the secretion amount of the catharanthine is also obviously increased. The synthetic culture medium realizes a good technical effect by adopting innovative technology improvement; meanwhile, the synthetic culture medium has relatively low cost and is easy to realize.

Description

technical field [0001] The invention relates to plant cell culture technology, and further relates to the design of nutritional conditions of plant cells, in particular to a synthetic medium for inducing vinca cells to secrete vinblastine. Background technique [0002] Plant cell culture technology is a culture process aimed at the expansion of cell mass and the accumulation of target metabolites based on isolated plant cells or protoplasts. For cell culture for the purpose of obtaining specific metabolites, the main process link is not only the expansion of cell mass, but also the induction of synthesis of target metabolites is more important for culture benefits. In the process of cultivating plant cells, culture conditions and nutritional conditions are the most important factors affecting yield. The culture conditions refer to the control of environmental conditions such as temperature, dissolved oxygen, light, and pH, while the nutritional conditions mainly refer to the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04C12P17/18
CPCC12N5/0025C12N2500/30C12N2500/32C12N2500/76C12N2501/30C12N2501/70C12P17/182
Inventor 夏雪城裘先临马丹
Owner 天津市博爱生物药业有限公司