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Plant starch synthesis associated protein IbAATP and encoding gene and application thereof

A plant starch and coding technology, applied in the direction of plant gene improvement, application, plant peptides, etc., can solve the problem of difficulty in breeding new sweet potato varieties with high quality, high yield, salt tolerance and drought resistance, limiting the utilization of sweet potato breeding resources and the free combination of parents, etc. question

Active Publication Date: 2015-10-07
CHINA AGRI UNIV
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Problems solved by technology

[0003] Sweet potato is a vegetatively propagated crop. The incompatibility of interspecific and intraspecific hybridization severely limits the utilization of resources and the free combination of parents in sweet potato breeding. Breeding practice shows that it is difficult to breed high-quality, high-yielding, salt-tolerant plants with conventional cross-breeding methods. New drought-resistant sweet potato varieties

Method used

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  • Plant starch synthesis associated protein IbAATP and encoding gene and application thereof
  • Plant starch synthesis associated protein IbAATP and encoding gene and application thereof
  • Plant starch synthesis associated protein IbAATP and encoding gene and application thereof

Examples

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Embodiment 1

[0065] Example 1. Obtaining and Identification of Transgenic Sweet Potatoes with Changes in Total Starch Content and Starch Quality

[0066] 1. Acquisition of IbAATP gene

[0067] The steps of obtaining the IbAATP gene are as follows:

[0068] 1. Acquisition of the template: Extract the total RNA of the young leaves of Xu 781 with a plant total RNA extraction kit (product of Tiangen Biochemical Technology (Beijing) Co., Ltd., catalog number DP432), and use the total RNA with PrimeScript TM 1st Strand cDNA Synthesis Kit (Bao Bioengineering (Dalian) Co., Ltd.) reverse transcribed the first strand cDNA.

[0069] 2. Synthesize degenerate primers DA-F and DA-R.

[0070] 3. After completing steps 1 and 2, use the cDNA obtained in step 1 as a template, and use the DA-F and DA-R synthesized in step 2 as primers to amplify by PCR to obtain EST sequence 1 of about 764 bp and sequence it. According to the sequence of EST sequence 1, primers 3GSP1 and 3GSP2 were designed and synthesiz...

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Abstract

The invention discloses a plant starch synthesis associated protein IbAATP and encoding genes and an application thereof. The plant starch synthesis associated protein IbAATP provided by the invention is (1), (2) or (3): (1) a protein of which an amino acid sequence is shown in a sequence 2 of a sequence table; (2) a fused protein obtained in a way that a label is connected to the N end or / and C end of the protein shown in the sequence 2 of the sequence table; (3) a plant starch synthesis associated protein obtained by the substitution and / or deletion and / or adding of the protein shown in (1) or (2) through one or several amino acid residues. Experiments show that the plant starch synthesis associated protein IbAATP and the encoding genes provided by the invention can increase the total content of plant starch and / or change the quality of the starch.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a plant starch synthesis-related protein IbAATP and its coding gene and application. Background technique [0002] Sweet potato (Ipomoea batatas (L.) Lam.) is an important food, feed and industrial raw material crop, and as a new energy plant in today's world, its status is particularly important. my country is the world's largest sweet potato producer, with an annual planting area of ​​3.482 million hectares, accounting for 43.0% of the world's total planting area, and its annual output accounts for 68.6% of the world's total output. my country is currently in a period of rapid development, and the demand for energy continues to increase, so finding new alternative energy sources will be a long-term strategic work. As a root crop, sweet potato is rich in starch, and starch is one of the important raw materials for industrial ethanol production. Therefore, through in-dep...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00
CPCC07K14/415C12N15/8245
Inventor 何绍贞王雁楠刘庆昌翟红李仁崑
Owner CHINA AGRI UNIV
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