Automatic focusing device and method

Abstract

The invention discloses an automatic focusing device and a method. The automatic focusing device adjusts the position of an objective lens via a first laser, a first optical fiber coupler, a second laser, a second optical fiber coupler, a third optical fiber coupler, a single-mode optical fiber, an alignment element, the objective lens, a semi-transmitting and semi-reflecting mirror, a relay mirror, a CCD / CMOS imaging device, a first photoelectric detector, a second photoelectric detector and a controller. Automatic focusing can be realized quickly and accurately, and the problems of multiple times of operation and slow focusing speed during a picture photographing process can be solved.

Description

technical field

[0001] The invention relates to the technical field of optical detection, in particular to an automatic focusing device and method. Background technique

[0002] In the field of cell research, real-time observation of cells is a very important link, including observing the shape and division of cells to study cell growth and cell activity.

[0003] In the laboratory, the cells used for research generally grow on the bottom surface of the culture box, and the cells are constantly moving. There is a difference in refractive index between the cells and the bottom surface of the culture box, and there is also a difference in refractive index between the outside air and the bottom surface of the culture box. Both are easy to cause reflections, so when observing cells and shooting cells, the autofocus of the shooting equipment needs to be accurate and fast.

[0004] At present, there are mainly two methods for automatic focusing of shooting equipment. One is to ta...

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