The invention discloses an automatic adjusting jig for focusing of a fixed-focus lens and an adjusting method of the automatic adjusting jig. The adjusting jig comprises a machine base, a rotating device, a driving device and a control device, wherein the control device has an electronic input interface used for acquiring image sharpness information of the lens and an electronic output interface that outputs a rotation direction and a rotation step length signal, the electronic output interface is connected to the driving device, the driving device and the rotating device are mounted on the machine base, the rotating device is provided with a mechanical rotating interface used for being connected with the lens, and the driving device is connected to the rotating device to drive the rotating device to rotate the fixed-focus lens according to the rotation direction and the rotation step length signal. Thus, the whole process does not need the participation of people, so that the workload of operating personnel is reduced, the production efficiency is greatly improved, artificial costs are reduced, the problem of a yield rate caused by visual fatigue of the operating personnel is prevented, and the yield rate is effectively improved.