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Chlorella pacifica medium

A technology of seawater chlorella and culture medium, applied in the field of culture medium, can solve the problems of blocking light, weakening photosynthesis, etc.

Active Publication Date: 2015-10-28
王兆伟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the autotrophic culture technology of Chlorella is mature, but with the continuous proliferation of cells, the light entering the culture is blocked, resulting in weakened photosynthesis, which limits the further increase of its biomass

Method used

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  • Chlorella pacifica medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A kind of seawater chlorella culture medium that the present invention proposes comprises the following components: NaNO 3 120mg / L, NaH 2 PO 4 12mg / L, ferric ammonium citrate 3.5mg / L, Na 2 EDTA 12mg / L, terbium chloride 0.5mg / L, brassinolide 0.0001mg / L, VB 1 0.006mg / L, VB 12 0.05mg / L, ZnSO 4 4H 2 O 23μg / L, MnCl 2 4H 2 O 178μg / L, CuSO 4 ·5H 2 O 10μg / L, Na 2 MoO 4 2H 2 O 7.3 μg / L, CoCl 2 ·6H 2 O 12 μg / L.

Embodiment 2

[0016] A kind of seawater chlorella culture medium that the present invention proposes comprises the following components: NaNO 3 120mg / L, NaH 2 PO 4 12mg / L, ferric ammonium citrate 3.5mg / L, Na 2 EDTA 12mg / L, terbium chloride 1mg / L, brassinolide 0.01mg / L, VB 1 0.006mg / L, VB 12 0.05mg / L, ZnSO 4 4H 2 O 23μg / L, MnCl 2 4H 2 O 178μg / L, CuSO 4 ·5H 2 O 10μg / L, Na 2 MoO 4 2H 2 O 7.3 μg / L, CoCl 2 ·6H 2 O 12 μg / L.

Embodiment 3

[0018] A kind of seawater chlorella culture medium that the present invention proposes comprises the following components: NaNO 3 100mg / L, NaH 2 PO 4 10mg / L, ferric ammonium citrate 2.5mg / L, Na 2 EDTA 10mg / L, terbium acetate 1mg / L, brassinolide 0.005mg / L, VB 1 0.006mg / L, VB 12 0.05mg / L, ZnSO 4 4H 2 O 23μg / L, MnCl 2 4H 2 O 178μg / L, CuSO 4 ·5H 2 O 10μg / L, Na 2 MoO 4 2H 2 O 7.3 μg / L, CoCl 2 ·6H 2 O 12 μg / L.

[0019] The medium obtained in Example 1 and Example 2 was used as the experimental group, and the seawater f / 2 medium was used as the control group. The seawater Chlorella pyrenoidosa in the logarithmic growth phase was inoculated into the experimental group and the control group respectively for cultivation, and the light was illuminated for 10 hours every day, and the light intensity was about 50 μmol / (m 2 s) The temperature of the incubator is controlled at 25±1°C, and the OD of Chlorella in the medium is recorded at regular intervals 700 and cell num...

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Abstract

The invention discloses a chlorella pacifica medium which contains the following components: 100-120 mg / L of NaNO3, 10-12 mg / L of NaH2PO4, 2.5-3.5mg / L of ammonium ferric citrate, 10-12 mg / L of Na2EDTA10, 0.5-1 mg / L of terbium salt, 0.0001-0.01 mg / L of brassinolide, 0.006mg / L of VB1, 0.05mg / L of VB12, 23 micrograms / L of ZnSO4.4H2O, 178 micrograms / L of MnCl2.4H2O, 10 micrograms / L of CuSO4.5H2O, 7.3 micrograms / L of Na2MoO4.2H2O and 12 micrograms / L of CoCl2.6H2O. During the culture process of chlorella, survival rate of chlorella is high, and growth rate of chlorella is fast.

Description

technical field [0001] The invention relates to the technical field of culture medium, in particular to a seawater chlorella culture medium. Background technique [0002] As an important microalgae resource, Chlorella is extremely rich in nutrients and excellent in health care. Its protein content is 50%-67%, which contains 20 kinds of amino acids, multivitamins and trace elements necessary for the human body, as well as linolenic acid, linoleic acid, carotene and other ingredients. Among them, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), these two components have been proved by clinical medicine to have various important physiological functions. Secondly, the polysaccharides and proteins contained in Chlorella have significant anti-tumor, anti-cancer, immune-enhancing and anti-viral activities. In aquaculture, single-cell chlorella can be directly used as bait for herbivorous fish and fry, or as bait for animals such as Artemia that are eaten by carnivorous...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 王兆伟
Owner 王兆伟
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