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Application of hemin in preparation of drugs used for preventing and treating muscle atrophy caused by sepsis

A technology of sepsis muscular atrophy and hemin, applied in the research field of sepsis muscular atrophy, can solve undiscovered problems

Inactive Publication Date: 2015-11-04
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, as the substrate and inducer of HO-1, there are no relevant studies and reports on the prevention and treatment of sepsis muscle atrophy using Hemin as the active ingredient.

Method used

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  • Application of hemin in preparation of drugs used for preventing and treating muscle atrophy caused by sepsis
  • Application of hemin in preparation of drugs used for preventing and treating muscle atrophy caused by sepsis
  • Application of hemin in preparation of drugs used for preventing and treating muscle atrophy caused by sepsis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1. Observation of the effect of Hemin on the degree of muscle atrophy in the hind limbs of CLP mice

[0039] By observing the degree of atrophy of the hindlimbs of the mice, it was determined whether Hemin can alleviate the reduction in the size of the lower limb muscles of the mice caused by sepsis. Under the premise that the size of the left / right paws of the mice in each group is basically the same, the degree of reduction in the volume of the hind limbs can reflect the degree of muscle atrophy.

[0040] experimental method

[0041] Step 1, 3 days after the operation, 6 mice were randomly selected from each experimental group, the experimental mice were inhaled sevoflurane to anesthetize the mice, and the mice were placed on the operating table in a supine position;

[0042] Step 2: Disinfect the hind left and right hind limbs of the mice with iodophor, cut the skin from the distal end to the proximal end along the inside of the mouse hind limbs, and bluntly...

Embodiment 2

[0046] Embodiment 2, the influence of Hemin on the wet weight change of CLP mouse soleus muscle

[0047] Weighing the soleus muscle wet weight is a common method to evaluate the degree of muscle atrophy. The wet weight of the soleus muscle can be continuously weighed after the operation to find the speed and trend of muscle atrophy, and to a certain extent, the dynamic effect of Hemin on muscle atrophy in sepsis can be evaluated.

[0048] experimental method:

[0049] Step 1: On the postoperative day, the first day, the third day, the fifth day and the seventh day, 6 experimental mice were randomly selected from each group, and after being anesthetized with sevoflurane, the experimental mice were fixed in a supine position on the console;

[0050] Step 2: Expose the mouse hindlimb muscles according to the method in Example 1, use the separating forceps to bluntly separate the soleus muscle along the tibia and fibula of the mouse, and separate the sections at the knee joint o...

Embodiment 3

[0053] Embodiment 3, the influence of Hemin on mouse soleus muscle protein catabolism

[0054] Accelerated muscle fiber protein degradation is the direct cause of muscle mass loss. Since tyrosine is neither synthesized nor decomposed in muscle, and tyrosine will not accumulate in the cytoplasm, the muscle can be analyzed indirectly by measuring the release rate of tyrosine in the culture medium after the muscle is cultured in vitro. protein degradation rate.

[0055] experimental method

[0056] Step 1, 24 hours after CLP, 6 mice were randomly selected from each experimental group, and the soleus muscle of the mice was obtained by the method in step 2 in Example 2;

[0057] Step 2, immerse the muscle tissue removed in step 1 into Krebs-Henseleit bicarbonate (KHB) buffer, incubate for 2 hours at 37°C under oxygenated conditions, take the culture solution, centrifuge at 4000g for 10 minutes, take 0.5mL supernatant, and mix with 1mL 0.5 % trichloroacetic acid and mix well, add...

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Abstract

The invention provides novel application of hemin. Experiments prove that hemin can inhibit secretion of proinflammatory factors in a mouse with sepsis, decrease the expression levels of Atrogin-1 and MuRF1 in muscle tissue E3 ubiquitin ligase and reduce the oxidative stress level of a body, thereby mitigating the degradation degree of muscle fiber protein and alleviating development of muscle atrophy; thus, hemin can be used as an effective component of drugs used for preventing or treating muscle atrophy caused by sepsis.

Description

technical field [0001] The invention belongs to the technical field of sepsis muscular atrophy research, and in particular relates to the application of hemin in the preparation of drugs for preventing and treating sepsis muscular atrophy. Background technique [0002] Muscular atrophy is a complication characterized by a decrease in skeletal muscle weight and volume and a decrease in the cross-sectional area of ​​muscle fibers in the presence of various diseases or abnormal physiological conditions, such as malnutrition, long-term bed rest, weightlessness, denervation, and advanced age. Muscular atrophy is often manifested clinically as a decrease or disappearance of muscle strength, which seriously affects the quality of life of patients. The etiology of muscle atrophy is complex and diverse, and its specific pathophysiological mechanism is still unclear. Accelerated muscle fiber protein degradation is currently recognized as one of the main mechanisms that cause muscle we...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/555A61P21/00
Inventor 薄禄龙于雄伟朱佳丽胡宝吉李金宝邓小明
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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