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A kind of sweet potato brown spot resistance identification method

A technology for resistance identification and brown spot disease, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the lack of identification methods for sweet potato brown spot disease resistance and the lack of research on brown spot disease resistance identification, etc. problems, to achieve the effects of easy control of test conditions, scientific identification methods, and simple operation

Inactive Publication Date: 2017-12-08
JIANGSU XUZHOU SWEET POTATO RES CENT
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many researches on the resistance to diseases such as sweet potato black spot, nematode disease and soft rot, but since sweet potato brown spot is a new disease of sweet potato in recent years, the identification of resistance of different sweet potato varieties to brown spot The research is quite scarce. At present, there is no standard method for identifying the resistance of sweet potato brown spot disease.

Method used

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  • A kind of sweet potato brown spot resistance identification method
  • A kind of sweet potato brown spot resistance identification method
  • A kind of sweet potato brown spot resistance identification method

Examples

Experimental program
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Embodiment

[0021] A method for identifying resistance to brown spot disease of sweet potato, we carry out the identification test of resistance to brown spot disease on the potato pieces of 16 sweet potato varieties collected during the harvest period, and carry out as follows:

[0022] 1. Isolation and preparation of pathogenic bacteria: The diseased potato pieces were collected from the experimental field of Xuzhou Sweet Potato Research Center, Chinese Academy of Agricultural Sciences, and the pathogenic bacteria were isolated by tissue isolation and single spore isolation, and confirmed by Koch's law. Cultivate it on a potato dextrose agar (PDA) plate for 7 days at 26°C, and set aside;

[0023] 2. Preparation of test potato pieces: when harvesting, select 16 sweet potato varieties, each sweet potato variety selects 3 medium-sized, smooth surface, no rotten potato pieces, carefully washes, dries, numbers, 75% Alcohol disinfection for use;

[0024] 3. Preparation of inoculum: wash down...

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Abstract

The invention discloses a method for identifying resistance to sweet potato brown spot disease, a new sweet potato disease, and belongs to a method for identifying resistance to crop diseases and insect pests. The method is scientific, the operation process is simple, and the requirements for the test conditions are not high. Select 3 medium-sized, smooth surface, and no rotten potato pieces to be identified, wash them carefully, dry them, number them, disinfect them with 75% alcohol, and inoculate them beforehand. In the brown spot spore suspension prepared with sterile water at a concentration of 1×106 / mL, sterilize wet gauze to keep it moist, and cultivate it at room temperature at 26°C for 10 days, investigate the damage diameter of each test tuber, and calculate the disease resistance The performance percentage, the resistance of each tested variety to sweet potato brown spot disease can be judged by the resistance performance percentage.

Description

technical field [0001] The invention relates to a method for identifying resistance to crop diseases, in particular to a method for identifying resistance to sweet potato brown spot disease caused by Fusarium, and belongs to the field of agricultural plant protection. Background technique [0002] Sweet potato brown spot, caused by the infection of Fusarium spp, is one of the main diseases of sweet potato during the growth period and storage period. It is a new disease that appeared in recent years, 2012-2015 The census of sweet potato diseases in 2010 found that the disease is widely distributed in all sweet potato production areas across the country. The disease mainly manifests as round, dark brown to black lesion spots on sweet potato pieces, which are slightly sunken and have a slightly bitter taste. The disease symptoms are similar to sweet potato black spot. It often co-occurs with other diseases, causing the whole cellar to rot and causing serious loss by extrusion....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04
Inventor 张成玲谢逸萍赵永强孙厚俊徐振杨冬静
Owner JIANGSU XUZHOU SWEET POTATO RES CENT
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