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Visual multicolor detection kit forantigen-antibody reaction and using method of kit

A technology of antibody reaction and kit, applied in the field of immune analysis and diagnosis, to achieve the effect of stable reaction system, rapid readout and low cost

Active Publication Date: 2015-11-18
NANJING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, immunosensing methods that can simultaneously detect multiple antigen-antibody responses with multi-color visualization are still in the exploratory stage.

Method used

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  • Visual multicolor detection kit forantigen-antibody reaction and using method of kit
  • Visual multicolor detection kit forantigen-antibody reaction and using method of kit
  • Visual multicolor detection kit forantigen-antibody reaction and using method of kit

Examples

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Embodiment 1

[0023] (1) Synthesis of three antibodies labeled with color-changing molecule functionalized graphene

[0024] Weigh 10 mg of commercially available nanometer graphene oxide (purchased from Nanjing Xianfeng Company), first add 10 mL of pure water, and ultrasonically disperse for 0.5 to 1 hour. Take 1mL of the above nano graphene oxide solution, first adjust the pH value to 6.0 with 0.5M sodium hydroxide, then add 4mg of N-hydroxysuccinimide and 2mg of 1-(3-dimethylaminopropyl)-3-ethyl carbon The diimine hydrochloride was shaken at room temperature for 20 minutes at a low speed, and the resulting reactant was centrifuged in a high-speed centrifuge at 13,000 rpm for 10 minutes, and the precipitate was dispersed in 0.7 mL of PBS buffer with a pH value of 8.0. Then the concentration was 0.1mg·mL -1 Add 200 μL of the antibody diluent solution to the treated nano carboxylated graphene oxide solution, shake at a low speed at 4°C for 6 hours, then add 100 μL of 5% BSA solution to the...

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Abstract

The invention belongs to a field of immunization analysis and diagnosis technology, and concretely relates to a visual multicolor detection kit for antigen-antibody reaction and a using method of kit. According to the invention, the kit comprises CEA antibodies labeled with grapheme modified by methyl red; NSE antibodies labeled with grapheme modified by phenolphthalein;Cyfra21-1 antibodies labeled with grapheme modified by thymolphthalein; a 96-well plate coated with a monoclonal antibody mixture containing CEA, NSE and Cyfra21-1 antibody; alkaline water whose pH value is 12.0; and a PBST buffersolution. The kit provided by the invention can be used for simultaneous multicolor detection of CEA, NSE and Cyfra21-1 antigen. The kit provided by the invention has the advantages of simple operation, high efficiency and low cost, and is convenient to be used in the places short of resource and less developed areas.

Description

1. Technical field [0001] The invention belongs to the technical field of immune analysis and diagnosis, and in particular relates to a visual multicolor detection kit for antigen-antibody reaction and a use method thereof. 2. Background technology [0002] ELISA is a technique in which known antigens or antibodies are adsorbed on the surface of a solid-phase carrier, and the enzyme-labeled antigen-antibody reaction is carried out on the solid-phase surface. This technology can be used to detect macromolecular antigens and specific antibodies, etc., and has the advantages of rapid response, sensitive detection, simple operation, low cost of use, and easy standardization of carriers. [0003] Although the enzyme-linked immunosorbent assay has many advantages, it can only detect one substance in a single color at a time due to the need to use enzyme-labeled antibodies to achieve signal transduction and output, which greatly limits the detection efficiency. Enzymes such as hor...

Claims

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Application Information

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IPC IPC(8): G01N33/53
CPCG01N33/53
Inventor 李根喜李超吴丹卫璐明黎天琪
Owner NANJING UNIV
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