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Sweet potato low-temperature-resistant related protein IbICE1 and coding genes and application thereof

A coding, protein technology, applied in the application, the use of vectors to introduce foreign genetic material, angiosperms/flowering plants, etc., can solve the problems of death, increased consumption, blocked energy production and material synthesis, etc.

Active Publication Date: 2015-11-25
LIAONING ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is generally believed that low temperature stress reduces photosynthesis of plants, enhances respiration, blocks energy production and material synthesis, increases consumption, and leaves plants in a state of starvation, which seriously affects the normal growth and development of plants and even leads to death.
Therefore, low temperature is one of the important abiotic stress factors that limit the growth and development of plants, determines the spatial and temporal distribution of plants, causes huge losses to agricultural production almost every year, and seriously affects the yield and quality of crops.

Method used

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  • Sweet potato low-temperature-resistant related protein IbICE1 and coding genes and application thereof
  • Sweet potato low-temperature-resistant related protein IbICE1 and coding genes and application thereof
  • Sweet potato low-temperature-resistant related protein IbICE1 and coding genes and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1、I

[0081] Embodiment 1, the acquisition of IbICE1 protein and its coding gene

[0082] 1. Extraction and purification of total RNA from leaves

[0083] About 2 g of the expanded leaves of Liaoshu 36 field seedlings were taken, ground into powder in liquid nitrogen, added to a 10 mL centrifuge tube, and the total RNA of sweet potato leaves was extracted with the Applygen Plant RNA Extraction Kit (Applygen Technologies Inc, Beijing).

[0084] mRNA was purified from total RNA using the QIAGEN ligotex Mini mRNA Kit (QIAGEN, GmbH, Germany). Take 1 μL of mRNA and check its integrity by electrophoresis on 1.2% agarose gel, and another 2 μL is diluted to 500 μL, and its quality (OD 260 ) and purity (OD 260 / OD 280), detected by non-denaturing agarose gel electrophoresis, the 28S and 18S bands were clear, and the brightness ratio of the two was 1.5-2:1, indicating that the total RNA was not degraded, and the purified mRNA met the experimental requirements and could be used for sweet po...

Embodiment 2

[0096] Embodiment 2, the acquisition of IbICE1 sweet potato plant and its low temperature resistance analysis

[0097] 1. Obtaining of sweet potato plants transfected with IbICE1

[0098] 1. Construction of recombinant vector pCAMBIA3301-IbICE1

[0099] (1) According to the coding sequence of the sweet potato IbICE1 protein cDNA, design and amplify the primer sequence of the complete coding sequence, the forward and reverse primers respectively introduce the BglII and PmlI restriction sites, and the primer sequences are as follows:

[0100] Primer 7: 5'- AGATCT ATGAACACACCAAGCCTAGCA-3' (the underlined part is the BglII restriction site);

[0101] Primer 8: 5'- CACGTG CTATACCAACCCATGGAAGGC-3' (the underlined part is the restriction site of PmlI).

[0102] (2) Using the DNA molecule shown in sequence 1 in the artificially synthesized sequence listing as a template (or using Liaoshu 36 cDNA as a template), PCR amplification was performed using primers 7 and 8 to obtain a PC...

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Abstract

The invention discloses sweet potato low-temperature-resistant related protein IbICE1 and coding genes and an application thereof. The sweet potato low-temperature-resistant related protein IbICE1 is one of protein in a), b) and c). The amino acid sequence of the protein a) is the same as that of the protein shown in the sequence 2 of a sequence table; the protein b) is the protein obtained by making the N end and / or the C end of the protein shown in the sequence 2 of the sequence table connected with labels; according to the protein c), one or more amino acid residues are replaced and / or lost and / or added to the amino acid sequence shown in the sequence 2 of the sequence table, and the protein with the same function is obtained. According to experiments, it is verified that the protein IbICE1 and the coding genes thereof play an important role in the process of resisting abiotikus stressz of plants, important application value is achieved in the study of improving the low temperature resistance of the plants, and wide application space and market prospect are achieved in the agriculture field.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a sweet potato low-temperature resistance-related protein IbICE1 and its coding gene and application. Background technique [0002] The low temperature and chilling injury of crops is one of the important disasters affecting China's grain production, and it occurs most frequently and seriously in the northern region, especially in the northeast region of my country. Chilling damage occurs every 8 years in the central part of Northeast China, and research on crop chilling damage is of great significance to China's food security. It is generally believed that low temperature stress reduces plant photosynthesis, enhances respiration, blocks energy production and material synthesis, increases consumption, and leaves plants in a state of starvation, which seriously affects the normal growth and development of plants and even leads to death. Therefore, low temperature is one o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C07K14/415C12N15/82A01H5/00C12N15/11
Inventor 于涛叶鑫张海楼
Owner LIAONING ACAD OF AGRI SCI
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