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Mercury-chelated immune complex, preparation method and application thereof

An immune complex and chelating technology, applied in the medical field, can solve problems such as functional changes, changes in the content of circulating immune complexes, and increases

Inactive Publication Date: 2015-12-09
广东腾湃医疗股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But how does environmental pollution stimulate the body to significantly increase the CIC content? Is it caused by one or multiple pollutants? Whether the increase in CIC will cause damage to the body and increase the susceptibility of the body to diseases? all to be studied
As we all know, circulating immune complexes are proteins of special significance, and mercury can act on proteins in multiple systems throughout the body. Whether mercury can act on circulating immune complexes will change the content, activity and function of circulating immune complexes , there is still a lack of relevant research on these

Method used

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  • Mercury-chelated immune complex, preparation method and application thereof
  • Mercury-chelated immune complex, preparation method and application thereof
  • Mercury-chelated immune complex, preparation method and application thereof

Examples

Experimental program
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preparation example Construction

[0062] Among them, the first preparation method includes

[0063] A) the step of synthesizing mercury chelated immune complex;

[0064] B) The step of purifying the mercury chelating immune complex: using immunoaffinity chromatography to remove the carrier protein, specific antibody and mercury ions that do not participate in the reaction in the mercury chelating immune complex synthesized in step A).

[0065] Specifically, in the preparation method of the mercury chelated immune complex,

[0066] (1) preparing chelating agent solution: chelating agent is dissolved, and is mixed with chelating agent solution;

[0067] (II) Prepare carrier protein solution: add carrier protein to borate buffer solution to prepare carrier protein solution;

[0068] (Ⅲ) Stir overnight: add the chelating agent solution in step (I) to the carrier protein solution in step (II), stir and act on a shaker for 2-30 hours to obtain a mixed solution;

[0069] (Ⅳ) Dialysis bag pretreatment: add EDTA-NaH...

Embodiment 1

[0164] In this example, ITCBE was purchased from Japan Tongren Chemical Research Institute, item number M030;

[0165] The following borate buffer solution, its preparation method example: Weigh 0.31g boric acid and dissolve it in 400mLddH 2 In O, use 0.1Mol / L NaOH aqueous solution to adjust the pH to 9.0, and set the volume to 500mL, to obtain;

[0166] The following EDTA-NaHCO 3 Solution preparation method example: take 1.86gEDTA·2H 2 O and 16.8 g NaHCO 3 , dissolved in 900mLddH 2 In O, adjust the pH to 8.0 with 1.0M NaOH and dilute to 1000mL, autoclave, and store at 25±2°C;

[0167] The following dialysis bags had a molecular weight cut-off of 14000 and were purchased from Bioshop Inc; the dialysis bags were processed as follows: the dialysis bags were placed in 500 mL volume of EDTA-NaHCO 3 solution, boil for 10min; discard EDTA-NaHCO 3 solution with ddH 2 Rinse with O, then boil with 500mL5mmol / LEDTA for 10min; discard the boiling liquid, thoroughly rinse with ddHO...

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Abstract

The invention discloses a mercury-chelated immune complex, a preparation method and an application thereof. The mercury-chelated immune complex is one from the following complexes: 1) a complex formed by combining mercury ion to an immune complex; 2) a complex formed by combining mercury ion to a carrier protein with an antibody capable of being specially combined with the carrier protein; or 3) a complex formed by combining mercury ion to an immune globulin and then combining the immune globulin with the carrier protein. The mercury-chelated immune complex has a relative specificity and is used for detecting whether human serum in people in a certain region contains mercury or not and detecting the content of mercury, thereby indirectly reflecting mercury pollution degree of this region.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a mercury chelated immune complex and a preparation method and application thereof. Background technique [0002] Immune complexes (IC) are the product of antigen-antibody binding. According to the definition of the National Committee for Scientific and Technical Terms, IC refers to the complex formed by the combination of antibody and soluble antigen. It is divided into two categories, one is the fixed immune complex in the tissue, and the other is the immune complex that is free in the blood and becomes the circulating immune complex (circulatingimmunecomplexes, CIC). Immune complexes play an indispensable role in clearing and destroying various antigens, and the result of the body's immune response is the continuous production of immune complexes. When the number of antigens (or antibodies) in the body is slightly more than that of antibodies (or antigens), an immune compl...

Claims

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Application Information

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IPC IPC(8): G01N33/531G01N33/53
CPCG01N33/53G01N33/531
Inventor 张积仁阳帆董欣敏吴婧蔡睿孙遥赵乙木
Owner 广东腾湃医疗股份有限公司
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