High-efficiency propagation method of Zinnia elegans virus-free seedling

A kind of technology of virus-free seedling and zinnia

Inactive Publication Date: 2015-12-23
陈丁龙
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the problems of low induction rate, poor quality of induced seedlings and many weak seedlings, low seedling proliferation efficiency, and low survival rate of transplanted seedlings in the current methods for the propagation of virus-free zinnia seedlings, the present invention provides a zinnia The high-effici

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  • High-efficiency propagation method of Zinnia elegans virus-free seedling
  • High-efficiency propagation method of Zinnia elegans virus-free seedling
  • High-efficiency propagation method of Zinnia elegans virus-free seedling

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Embodiment 1

[0047] In this embodiment, the method for the efficient multiplication of detoxified zinnia seedlings, the experimental variety was selected as Fangfei No. 2, and the implementation time was 2014-2015. The city revitalizes the flower garden, and the tissue culture seedlings are transplanted in the flower test base of Dongxin Farm, Lianyungang City. The detailed steps are as follows:

[0048] 1) Pre-treatment of Fangfei No. 2 virus-free seedlings:

[0049] Rinse Fangfei No. 2 virus-free seedlings with tap water repeatedly first, wash once with a brush dipped in detergent, then rinse with ultra-pure water, soak in 70% alcohol for 15 seconds, and then rinse twice with sterile water, 0.1% Add a few drops of Tween 20 solution to the mercuric acid and soak for 5 minutes, rinse with sterile water 4-5 times, blot dry with filter paper, and transfer the petiole and receptacle of Fangfei No. 2 virus-free seedlings into adventitious bud induction medium (the formula is: MS+0.5mg / LKT+1.5...

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Abstract

The invention discloses a high-efficiency propagation method of Zinnia elegans virus-free seedlings. The high-efficiency propagation method of the Zinnia elegans virus-free seedlings comprises the following steps: 1) performing Zinnia elegans virus-free seedling pretreatment; 2) inducing adventitious buds; 3) cutting off the adventitious buds for subculture multiplication; 4) hardening seedlings; 5) performing water culture rooting treatment; 6) performing water culture seedling strengthening; 7) transplanting the seedlings. The high-efficiency propagation method disclosed by the invention solves the problems that the efficiency is low and the seedlings are easily polluted again during Zinnia elegans virus-free seedling propagation, not only can a great amount of Zinnia elegans virus-free seedlings be propagated in short time, but also the problem of variety degeneration caused by Zinnia elegans division propagation and seed propagation can be avoided, the operation is simple, the factory popularization is facilitated and the practical production value is very great.

Description

technical field [0001] The invention relates to a high-efficiency multiplication method for virus-free zinnia seedlings, in particular to a multiplication method capable of producing a large number of high-quality anti-virus zinnia seedlings in a short period of time, and belongs to the technical field of horticultural cultivation. Background technique [0002] Zinnia, Asteraceae, Zinnia genus, native to North America, Mexico and South America and other places, because of rising steadily, the flowers bloom more and more luxuriantly, and have won many good names, such as Zhuangyuanhong, Huoqiuhua, Zinnia, Backgammon and more. Zinnia likes warmth, is not cold-resistant, and is resistant to drought. It should be sunny, and the tongue-shaped flowers will increase under long-day conditions. Zinnia has a long flowering period and a large amount of flowers. Each plant blooms 30-40 flowers from the first flower to the frost. Most of the excellent varieties are Melaleuca petals, wh...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00
Inventor 不公告发明人
Owner 陈丁龙
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