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Tissue culture type gastrodia elata seed breeding technology

A tissue culture and gastrodia elata technology, applied in horticulture, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems of low survival rate, easy disease and disability, low yield and low value, etc., to achieve high survival rate, economical High-value, high-yield effects

Inactive Publication Date: 2015-12-23
CHENGDU GAOSHANWU RHIZOMA GASTRODIAE PLANTATION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention proposes a tissue culture Gastrodia elata seed breeding technology, which can solve the problems of low survival rate, easy disease and disability, low yield and low value, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Select 4kg of soybeans, 4kg of corn, 2kg of sawdust, and fresh logs with a diameter of 7cm, and each tissue culture unit is soaked in warm water with a length of 3cm to make it absorb enough water;

[0010] Get 3kg of bran, 4kg of potato slices, 1kg of gypsum powder, boil water together, get 96L of filtrate, then add 2kg of brown sugar and 2kg of glucose to make a liquid culture material;

[0011] Mix soybeans, corn, and sawdust on the bottom of the tissue culture bottle to a thickness of 4 cm, and then put the log in, so that the log is submerged by 2 cm, leaving 1 cm to expose the top surface of the soybean, corn, and sawdust ingredients. Add liquid culture material until it is 1 cm away from the top surface of the log. , cover with cotton-free strains to make medium I; add water to sawdust and river sand respectively to adjust the water content to 65%, and pack them into bags to tie the bag mouths to make culture supplements II and III; High temperature and high pres...

Embodiment 2

[0016] Select 2kg of soybean, 2kg of corn, 1kg of sawdust, and fresh logs with a diameter of 7cm, and each tissue culture unit is soaked in warm water with a length of 3cm to make it absorb enough water;

[0017] Get 3kg of bran, 4kg of potato slices, 1kg of gypsum powder, boil water together, get 96L of filtrate, then add 2kg of brown sugar and 2kg of glucose to make a liquid culture material;

[0018] Mix the soybeans, corn and sawdust on the bottom of the plastic bag to a thickness of 4cm, then put the log in, so that the log is submerged by 2cm, leaving 1cm to expose the upper surface of the soybean, corn and sawdust ingredients, and add the liquid culture material until it is 1cm away from the upper surface of the log. Tighten the mouth of the bag with a rope to form culture medium I; add water to the sawdust and river sand respectively to adjust the water content to 60%, and pack the bag to tie the mouth of the bag to make culture supplements II and III; High temperature...

Embodiment 3

[0023] Select 2kg of soybean, 2kg of corn, 1kg of sawdust, and fresh logs with a diameter of 7cm, and each tissue culture unit is soaked in warm water with a length of 3cm to make it absorb enough water;

[0024] Get 3kg of bran, 4kg of potato slices, 1kg of gypsum powder, boil water together, get 96L of filtrate, then add 2kg of brown sugar and 2kg of glucose to make a liquid culture material;

[0025] Mix the soybeans, corn and sawdust on the bottom of the plastic bag to a thickness of 4cm, then put the log in, so that the log is submerged by 2cm, leaving 1cm to expose the upper surface of the soybean, corn and sawdust ingredients, and add the liquid culture material until it is 1cm away from the upper surface of the log. Tighten the mouth of the bag with a rope to form culture medium I; add water to the sawdust and river sand respectively to adjust to 70% water content, and pack the bag to tie the mouth of the bag to make culture supplements II and III; High temperature and...

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Abstract

The invention provides a tissue culture type gastrodia elata seed breeding technology. The tissue culture type gastrodia elata seed breeding technology is characterized by comprising the steps of preparing materials, preparing a liquid culture material, making a culture medium I and auxiliary culture materials II and III, performing sterilization, obtaining seed-dressing germination strains IV, inoculating armillaria strains, adding sterile sawdust II, spreading a layer of seed-dressing germination strains IV or evenly distributing 5-8 small disinfected and sterilized generation-0 gastrodia elata seeds, filling aseptic river sand III on a culture unit to make the single sealed culture unit V and then performing culture. The gastrodia elata seeds are bred in a tissue culture mode, all the raw materials are scientifically combined, the advantages of indoor stereo culture and breeding are fully utilized, and a modern scientific technique is applied to all links of seed breeding. The seeds cultured under the aseptic condition do not infect bacteria or infectious microbes. Due to the fact that breeding is performed indoors, damage caused by insect pests, rats and other animals can be avoided. The culture materials are full of nutriments, and cultured seeds are large and full. Due to high yield and high quality, high economic value is certainly produced, and a solid foundation is laid for further production.

Description

technical field [0001] The invention relates to the field of Gastrodia elata planting, in particular to a tissue culture type Gastrodia elata seed breeding technology. Background technique [0002] Gastrodia elata, also known as Chijian, Dingfengcao, Limu, Bailongpi, and Mingming Ma, is a precious Chinese medicinal material. Gastrodia elata is sweet, pungent, calming, non-toxic, enters the liver meridian, can relieve wind and stop spasm, calm liver-yang, expel wind and clear collaterals, and has the effect of treating convulsions, convulsions, dizziness, headache, hemiplegia, limb numbness, rheumatic arthralgia and other effects. Gastrodia is a perennial herbaceous parasite of the Orchidaceae family. It has no roots and no leaves. The plant does not contain chlorophyll, so it cannot perform photosynthesis and is autotrophic. It cannot directly absorb nutrients from the outside world. The bacteria provide the nutrients needed for the growth of Gastrodia elata. The seeds of...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G1/04
Inventor 徐贤锋
Owner CHENGDU GAOSHANWU RHIZOMA GASTRODIAE PLANTATION CO LTD
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