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Method for simultaneously determining contents of 10 chemical components in fourstamen stephania root and astragalus membranaceus decoction preparation by UHPLC-MS/MS (Ultra High Performance Liquid Chromatography-Mass Spectrograph)

A technology for the chemical composition of Fangji Huangqi Decoction, applied in measuring devices, scientific instruments, material separation, etc., can solve the problems of inability to measure the content, complex chemical composition, and little research on the chemical composition of Fangji Huangqi Decoction, etc., achieve short analysis period, High separation efficiency and high sensitivity

Active Publication Date: 2015-12-23
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

Although Fangji Huangqi Decoction has significant curative effect in clinical application, due to its complex chemical composition, so far there are few studies on the chemical composition of Fangji Huangqi Decoction
The main components of Fangji Huangqi Decoction include alkaloids, flavonoids, lactones, saponins, etc. Due to the limitation of the detector, the traditional detection technology HPLC-DAD cannot detect the multiple types of chemical structures in Fangji Huangqi Decoction at the same time. Simultaneous content determination of components

Method used

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  • Method for simultaneously determining contents of 10 chemical components in fourstamen stephania root and astragalus membranaceus decoction preparation by UHPLC-MS/MS (Ultra High Performance Liquid Chromatography-Mass Spectrograph)
  • Method for simultaneously determining contents of 10 chemical components in fourstamen stephania root and astragalus membranaceus decoction preparation by UHPLC-MS/MS (Ultra High Performance Liquid Chromatography-Mass Spectrograph)
  • Method for simultaneously determining contents of 10 chemical components in fourstamen stephania root and astragalus membranaceus decoction preparation by UHPLC-MS/MS (Ultra High Performance Liquid Chromatography-Mass Spectrograph)

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Embodiment 1

[0021] Example 1: Method for simultaneous determination of 10 chemical components in Fangji Huangqi Decoction by UHPLC-MS / MS:

[0022] 1. Decoction pieces, instruments and reagents

[0023] 1.1 Source of decoction pieces: Astragalus membranaceus and Radix Glycyrrhiza were purchased from Nanjing Haichang Traditional Chinese Medicine Group (Nanjing, China); Fenfangji and Baizhu were purchased from Hebei Traditional Chinese Medicine Group (Hebei, China); they were all genuine products after being identified by Professor Chen Jianwei, School of Pharmacy, Nanjing University of Traditional Chinese Medicine .

[0024] 1.2 Instruments

[0025] Shimadzu CBM20Alite high performance liquid chromatography system (ShimadzuLC20ADXR pump, SPD20AUV detector, SIL20ACXR autosampler, CT020AC column thermostat); TripleQuadrupole5500 mass spectrometer (ABSCIEX, USA), ESI ion source; mass spectrometry workstation: Analyst TF 1.6; Ultrasonic cleaner (KQ-500, Kunshan Ultrasonic Instrument Co., Ltd....

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Abstract

The invention relates to a method for simultaneously determining the contents of 10 chemical components in a fourstamen stephania root and astragalus membranaceus decoction preparation by UHPLC-MS / MS (Ultra High Performance Liquid Chromatography-Mass Spectrograph) and belongs to the field of analysis of Chinese herbal medicinal ingredients. An established analysis method for simultaneously determining the contents of the 10 chemical components in the fourstamen stephania root and astragalus membranaceus decoction preparation has strong specificity and is rapid, flexible, accurate and reliable; and the content determination can be finished while mass number information of compounds also can be accurately given, so that the effect of identifying the components is realized; and the method can provide evidences for total quality control of the fourstamen stephania root and astragalus membranaceus decoction preparation.

Description

technical field [0001] The invention relates to a method for simultaneously measuring the contents of 10 chemical components in Fangji Huangqi Decoction by using UHPLC-MS / MS technology, and belongs to the field of component analysis of traditional Chinese medicines. Background technique [0002] Fangji Huangqi Decoction is a commonly used traditional Chinese medicine prescription with significant curative effect in clinical practice. It is mainly used in the treatment of chronic nephritis proteinuria, rheumatoid arthritis, frozen shoulder, etc. Although Fangji Huangqi Decoction has significant curative effect in clinical application, due to its complex chemical composition, there are few studies on the chemical constituents of Fangji Huangqi Decoction. The main components of Fangji Huangqi Decoction include alkaloids, flavonoids, lactones, saponins, etc. Due to the limitation of the detector, the traditional detection technology HPLC-DAD cannot detect the multiple types of c...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 刘晓蔡皓蔡宝昌汪小莉祝婷婷
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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