Application of long-chain non-coding RNA (ribonucleic acid) in treatment of DDH (developmental dysplasia of hip) disease

A long-chain non-coding, dysplasia technology, applied in the field of application of long-chain non-coding RNAH19 in developmental dysplasia of the hip

Active Publication Date: 2015-12-30
XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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Problems solved by technology

However, the application of long non-coding RNAH19 (LncRNAH19) in developmental dysplasia of the hip (DDH) has not been reported yet.

Method used

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  • Application of long-chain non-coding RNA (ribonucleic acid) in treatment of DDH (developmental dysplasia of hip) disease
  • Application of long-chain non-coding RNA (ribonucleic acid) in treatment of DDH (developmental dysplasia of hip) disease
  • Application of long-chain non-coding RNA (ribonucleic acid) in treatment of DDH (developmental dysplasia of hip) disease

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Experimental program
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Embodiment 1

[0026] One: Materials and methods:

[0027] 1.1 Materials

[0028] 1.1.1 From April 2015 to August 2015, 7 cases of DDH patients and 6 cases of femoral neck fracture patients were collected from Xinhua Hospital Affiliated to Shanghai Jiaotong University, including 8 males and 5 females, age 31-76 years old, with an average of 51.1±14.4 years old. This study was approved by the Hospital Medical Ethics Committee and the patients gave informed consent.

[0029] 1.1.2 SD rats are provided by Shanghai Slack Experimental Animal Co., Ltd., male or female, with a body weight of 200g±15g.

[0030] 1.1.3 Main materials and reagents DMEM / F12 medium, fetal bovine serum FBS and penicillin-streptomycin double antibody solution (hyClone, USA); EDTA-trypsin (Gibco, USA); DMSO (Sigma, USA), BCA protein Concentration determination kit (Pierce, USA); NF-κB antibody kit and MAPK antibody kit (Cell Signaling Technology, USA).

[0031] 1.1.4 Rat primary chondrocytes: 5 SD rats were killed by ne...

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Abstract

The invention relates to a novel application of Lnc RNA H19 (long non-coding ribonucleic acid H19), that is, overexpression of Lnc RNA H19 can delay the progression of disease from DDH (developmental dysplasia of hip) to osteoarthritis. The application has the advantages that the research of 7 cases of DDH cartilage tissue and 6 cases of cartilage tissue of fractured femoral neck shows that the expression of Lnc RNA H19 in DDH is remarkably reduced compared with that of Lnc RNA H19 in femoral neck fracture, and H19 is possible to have a relation with occurrence and development of DDH; DDH is accelerated to deteriorate into osteoarthritis if H19 is knocked down; the expression of H19 can be promoted through transfection of a slow virus LV-H19, and the overexpression of H19 can delay the progression of disease from DDH to osteoarthritis.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to the application of long-chain non-coding RNAH19 (LncRNAH19) in developmental dysplasia of the hip (DDH). Background technique [0002] Long non-coding RNA (long non-coding RNA, lncRNA) is defined as a class of RNA transcripts longer than 200 nt that do not encode proteins. It was originally considered to be the "noise" of genome transcription, just a by-product of RNA polymerase II transcription. Has biological functions. However, studies in recent years have shown that lncRNAs are involved in many important regulatory functions such as X chromosome silencing, genome imprinting and chromatin modification, transcriptional activation, transcriptional interference, nuclear transport, protein post-translational modification, and epigenetic regulation of genetics. process. These effects indicate that it is closely related to the pathophysiological changes of diseases, espe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K31/7088A61P19/02G01N33/68
Inventor 陈晓东王成龙王晖朱俊峰沈超左斌李德肖飞
Owner XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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