Preparation method of organic fertilizer for bamboo forest and use method of organic fertilizer

Abstract

The invention provides a preparation method of an organic fertilizer for a bamboo forest. According to the method, plant litter is selected to acquire a nutrient solution, dominant bacteria of a plant litter layer are acquired after the separation, purification, culturing and breeding processes of bacteria, the acquired dominant bacteria are inoculated into compost prepared from the plant litter to be fermented, and the organic fertilizer for the forest is obtained. With the adoption of the organic fertilizer for the bamboo forest, the physical structure of soil can be changed, soil hardening can be improved, moisture and fertility are preserved, the soil fertility is improved, the number of beneficial microorganisms in soil is increased, harmful infectious microbes in the soil are killed, and accordingly, growth of root systems of bamboo is promoted. The invention further provides a use method of the organic fertilizer for the bamboo forest. With the adoption of the use method of the organic fertilizer for the bamboo forest, the bamboo shooting rate of the bamboo forest can be increased by 27.5%, the bamboo rate is increased by 10%, and the growth speed of young bamboo is increased by 15%.

Description

technical field

[0001] The invention relates to the technical field of organic fertilizer preparation, and more specifically, relates to a method for preparing and using an organic fertilizer for bamboo forests. Background technique

[0002] Bamboo belongs to Gramineae and is an important forest plant in tropical and subtropical regions of my country. Bamboo has been paid more and more attention by countries all over the world because of its special growth advantages and important economic value. Simultaneously because bamboo has higher ornamental value, thereby the cultivation of bamboo forest also more and more causes people's attention. According to statistics, my country's existing bamboo forest area is about 7.2 million hectares, of which the area of ​​artificially managed bamboo forests reaches 4.2 million hectares. ornamental value. Therefore, when artificially cultivating bamboo forests, how to improve the shoot rate, growth rate and forestation rate of bamboo fore...

Examples

Embodiment 1

[0031] S101: collecting bamboo forest litter with fungi in the litter layer of a healthy bamboo forest;

[0032] S102: cutting the collected litter of the bamboo forest into small pieces;

[0033] S103: Inoculate the litter and leaves of the bamboo forest cut into small pieces into the improved PDA plate medium, and cultivate them at a temperature of 22° C. until colonies grow;

[0034] S104: Select the dominant colony, transfer the hyphae at the edge of the dominant colony to a new improved PDA plate medium and continue culturing until pure mycelium grows;

[0035] S105: selecting vigorously growing pure mycelium, inoculating the pure mycelium into a modified PDA liquid medium for cultivation, and obtaining a liquid bacterial agent;

[0036] S106: inoculating the above-mentioned liquid bacterial agent into the solid compost to continue culturing to obtain a solid bacterial agent;

[0037] S107: Inoculate the above-mentioned solid bacterial agent into the expanded culture ma...

Embodiment 2

[0040] S201: collecting bamboo forest litter with fungi in the litter layer of healthy bamboo forest;

[0041] S202: cutting the collected litter of the bamboo forest into small pieces;

[0042] S203: Inoculate the litter and leaves of the bamboo forest cut into small pieces into the improved PDA plate medium, and cultivate them at a temperature of 26° C. until colonies grow;

[0043] S204: Select the dominant colony, transfer the hyphae at the edge of the dominant colony to a new improved PDA plate medium and continue culturing until pure mycelium grows;

[0044] S205: selecting vigorously growing pure mycelium, inoculating the pure mycelium into a modified PDA liquid medium for cultivation, and obtaining a liquid microbial agent;

[0045] S206: Inoculate the above-mentioned liquid bacterial agent into the solid compost to continue culturing to obtain a solid bacterial agent;

[0046]S207: Inoculate the above-mentioned solid bacterial agent into the expanded culture materia...

Embodiment 3

[0049] S301: collecting bamboo forest litter with fungi in the litter layer of a healthy bamboo forest;

[0050] S302: cutting the collected litter of the bamboo forest into small pieces;

[0051] S303: Boil bamboo litter and potatoes in boiling water for 20 minutes each, and filter to obtain bamboo litter boiling liquid with a concentration of 150 g / L and potato juice with a concentration of 150 g / L; boil the filtered bamboo litter Mix liquid, water and potato juice according to the volume ratio of 1:4:3 to obtain a mixed solution; add agar to the mixed solution, and stir while heating until the agar is completely melted, add glucose after the agar is completely melted, and stir evenly, wherein , the mass ratio of potato, glucose and agar is 15:1:1; after stirring evenly, use 0.1mol / L HCl and 0.1mol / L NaOH to adjust the pH value to 5, and carry out sterilization treatment; Cool to obtain the improved PDA plate medium. Inoculate the litter and leaves of the bamboo forest cut...

technical field

[0001] The invention relates to the technical field of organic fertilizer preparation, and more specifically, relates to a method for preparing and using an organic fertilizer for bamboo forests. Background technique

[0002] Bamboo belongs to Gramineae and is an important forest plant in tropical and subtropical regions of my country. Bamboo has been paid more and more attention by countries all over the world because of its special growth advantages and important economic value. Simultaneously because bamboo has higher ornamental value, thereby the cultivation of bamboo forest also more and more causes people's attention. According to statistics, my country's existing bamboo forest area is about 7.2 million hectares, of which the area of ​​artificially managed bamboo forests reaches 4.2 million hectares. ornamental value. Therefore, when artificially cultivating bamboo forests, how to improve the shoot rate, growth rate and forestation rate of bamboo fore...

Examples

Embodiment 1

[0031] S101: collecting bamboo forest litter with fungi in the litter layer of a healthy bamboo forest;

[0032] S102: cutting the collected litter of the bamboo forest into small pieces;

[0033] S103: Inoculate the litter and leaves of the bamboo forest cut into small pieces into the improved PDA plate medium, and cultivate them at a temperature of 22° C. until colonies grow;

[0034] S104: Select the dominant colony, transfer the hyphae at the edge of the dominant colony to a new improved PDA plate medium and continue culturing until pure mycelium grows;

[0035] S105: selecting vigorously growing pure mycelium, inoculating the pure mycelium into a modified PDA liquid medium for cultivation, and obtaining a liquid bacterial agent;

[0036] S106: inoculating the above-mentioned liquid bacterial agent into the solid compost to continue culturing to obtain a solid bacterial agent;

[0037] S107: Inoculate the above-mentioned solid bacterial agent into the expanded culture ma...

Embodiment 2

[0040] S201: collecting bamboo forest litter with fungi in the litter layer of healthy bamboo forest;

[0041] S202: cutting the collected litter of the bamboo forest into small pieces;

[0042] S203: Inoculate the litter and leaves of the bamboo forest cut into small pieces into the improved PDA plate medium, and cultivate them at a temperature of 26° C. until colonies grow;

[0043] S204: Select the dominant colony, transfer the hyphae at the edge of the dominant colony to a new improved PDA plate medium and continue culturing until pure mycelium grows;

[0044] S205: selecting vigorously growing pure mycelium, inoculating the pure mycelium into a modified PDA liquid medium for cultivation, and obtaining a liquid microbial agent;

[0045] S206: Inoculate the above-mentioned liquid bacterial agent into the solid compost to continue culturing to obtain a solid bacterial agent;

[0046]S207: Inoculate the above-mentioned solid bacterial agent into the expanded culture materia...

Embodiment 3

[0049] S301: collecting bamboo forest litter with fungi in the litter layer of a healthy bamboo forest;

[0050] S302: cutting the collected litter of the bamboo forest into small pieces;

[0051] S303: Boil bamboo litter and potatoes in boiling water for 20 minutes each, and filter to obtain bamboo litter boiling liquid with a concentration of 150 g / L and potato juice with a concentration of 150 g / L; boil the filtered bamboo litter Mix liquid, water and potato juice according to the volume ratio of 1:4:3 to obtain a mixed solution; add agar to the mixed solution, and stir while heating until the agar is completely melted, add glucose after the agar is completely melted, and stir evenly, wherein , the mass ratio of potato, glucose and agar is 15:1:1; after stirring evenly, use 0.1mol / L HCl and 0.1mol / L NaOH to adjust the pH value to 5, and carry out sterilization treatment; Cool to obtain the improved PDA plate medium. Inoculate the litter and leaves of the bamboo forest cut...

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