Station for cryopreserving cells

Abstract

The invention discloses an operating table for freezing and storing cells. The operating table includes an operating table, an incubator, a liquid nitrogen storage box, a cell freezing storage box and a self-pressurized liquid nitrogen tank; grooves are arranged on the operating table , an incubator is set in the groove, a liquid nitrogen storage box is set in the incubator, a cell cryopreservation box is set in the liquid nitrogen storage box, and one or more holes are set on the outer layer of the cell cryopreservation box, self-pressurized liquid nitrogen The tank is connected to the liquid nitrogen storage box; the cell freezing box is used to accommodate the cell freezing box, and the self-pressurized liquid nitrogen tank is used to provide liquid nitrogen to the liquid nitrogen storage box. The present invention provides a continuous and stable ultra-low temperature environment for storing cells, and also provides an ultra-low temperature environment during the process of extracting and processing cells, thereby effectively preventing the formation of ice crystals in the cells and causing the decline in cell activity or even death.

Description

technical field [0001] The invention relates to the field of cell storage, in particular to an operating platform for freezing cells. Background technique [0002] The main experimental objects of cell biology research are various primary cultured or subcultured cells or cell lines. In order to avoid the pollution caused by the cultured cells in the long-term in vitro culture process or the gene mutation caused by multiple passages, the experimenter will freeze the cultured cells during the vigorous growth period, and store the frozen cells in a container with cell freezing solution The cells are frozen in tubes, and then placed in liquid nitrogen at -196°C. In this ultra-low temperature environment, the cells can be stored for a long time. When the experiment is needed, the experimenter can revive the frozen cells at 37°C for the required experiment, which can ensure the genetic stability of the cells to the greatest extent. [0003] Cell biology studies have shown that t...

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to overcome the defect that the operating table in the prior art cannot provide an ultra-low temperature environment for accessing cells, so that ice crystals are formed in the cells, resulting in a decrease in cell activity or even death, and to provide a method for freezing and storing cells. console

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