Cosmetic plant polysaccharide composition
A technology of plant polysaccharides and compositions, applied in the direction of cosmetics, cosmetic preparations, dressing preparations, etc., can solve the problems of premature aging of the skin, degradation of metabolic functions, etc., to achieve enhanced metabolic functions, bright and beautiful skin, and good cosmetic activity effect of effect
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Embodiment 1
[0048] Preparation of the cosmetic composition:
[0049] 1. Mixture 1: prepared by mixing centella asiatica cell homogenate and vegetable glycerin, wherein the centella asiatica cell homogenate accounts for 20% by mass of the mixture 1.
[0050] 2. Mixture 2: prepared by mixing vegetable glycerin, centella asiatica cell homogenate and snow lotus flower extract. Wherein, the centella asiatica cell homogenate accounts for 10% by mass of the mixture 2, and the mass percent of the snow lotus flower extract accounts for 10% of the mixture 2 by mass.
[0051] 3. Mixture 3: prepared by mixing vegetable glycerin, centella asiatica cell homogenate, snow lotus flower extract, and American ginseng extract. Wherein, the centella asiatica cell homogenate accounts for 5% by mass of the mixture 3, the extract of snow lotus flower accounts for 20% by mass of the mixture 3, and the extract of American ginseng accounts for 5% by mass of the mixture 3.
[0052] 4. Mixture 4: It is prepared by ...
Embodiment 2
[0056] by CO 2 Effects of release assay mixtures on energy metabolism and physiological mechanisms in reconstructed skin Impact
[0057] Mix mixtures 1 to 6 with the experimental amount of reconstituted skin surface material required for ordinary in vitro tests, and measure the activity of cell metabolism. The ability of metabolism and oxidation is determined by the D-( 14 C) the metabolic capacity and 14 CO 2 determined by quantitative analysis.
[0058] Human keratinocytes were filtered through 0.5 cm2 polycarbonate filters and cultured in supplemented medium (modified MCDB153 medium). The cell culture process was carried out at the air / liquid interface, and the culture time was 14 days, and the medium was replaced every 2 days. The epidermis obtained by culturing on the 14th day in this embodiment.
[0059] In order to facilitate the evaluation of the activity of the mixture, the test should be carried out under certain airtight conditions, that is, to create a dilut...
Embodiment 3
[0074] Evaluate the effect of mixtures on the energy metabolism of cultured cells
[0075] ATP, ADP and AMP quantitative analysis methods were used to measure the energy carrying capacity of the cells after the test treatment, and the unit was nanomoles per mg of protein.
[0076] Specifically, the keratinocytes were mixed with mixture 1, mixture 4, mixture 5 and mixture 6 of different concentrations, and then placed in a 6-well cell culture plate for culture. The culture medium was KGM medium, in which insulin and Cortisol, wherein the content of keratinocytes in each culture medium is 10 6 , and the test lasted for 5 days. The keratinocytes are complete impermeable cells (purchased from EUROTEST).
[0077] After the experiment, the components in the medium were trypsinized, and the concentrations of ATP, ADP and AMD were determined by HPLC. Wherein, the CE energy carrying capacity is calculated by the following formula:
[0078] CE=([ATP]+0.5x[ADP]) / ([ATP]+[ADP]+[AMP])) ...
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