Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method of utilizing oxidant to treat microalgae to efficiently induce neutral lipid for quick accumulation

A technology of oxidant and neutral lipid, which is applied in the fields of microalgae and bioenergy, can solve problems such as water pollution, and achieve the effects of improving productivity, high efficiency and productivity, and high induction efficiency

Inactive Publication Date: 2016-02-10
INST OF AQUATIC LIFE ACAD SINICA
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The way of adding some special ingredients to induce due to the addition of some substances may cause water pollution to a certain extent

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method of utilizing oxidant to treat microalgae to efficiently induce neutral lipid for quick accumulation
  • Method of utilizing oxidant to treat microalgae to efficiently induce neutral lipid for quick accumulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for efficiently inducing rapid accumulation of neutral lipids by treating microalgae with oxidants, the steps are (taking 1L culture scale as an example):

[0023] 1. Chlorella autotrophic culture:

[0024]The sterile BG11 liquid medium was added to six 2L sterile Erlenmeyer flasks respectively, and the added volume was 1L to prepare six BG11 liquid medium. The purified Chlorella sp.C2 (Chen, H., Zhang, Y.M., He, C.L., Wang, Q., 2014. Plant & Cell Physiology, 55: 634-644) was inoculated on six BG11 liquid medium, the seeding density is OD 700 =0.05 (about 1.1×10 6 cells / mL), and finally at the same time at a temperature of 25 °C and a light intensity of 70 μmolm -2 s -1 cultured under continuous aeration conditions.

[0025] 2. Oxidant-induced treatment

[0026] In this method, a standard curve between OD value and biomass is established with 700 nm as the scanning wavelength, and the concentration of biomass in the liquid medium is calculated by measurin...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method of utilizing an oxidant to treat microalgae to efficiently induce neutral lipid for quick accumulation. The method includes: disposing the microalgae in an autotrophic culture medium for conventional autotrophic culture; when the microalgae are in the middle stage of logarithmic growth, starting to slowly adding a solution containing the oxidant H2O2, Bengal rose, peroxynitrite, decadienal or methyl viologen dropwise, and inducing accumulation of neutral lipid of microalgae cells, wherein content of the neutral lipid in the microalgae cells can be made equal to or higher than that of the neutral lipid generated by using other induction methods for treatment through induced culture for only 24-48h. The method is simple and easy to implement, and the oxidant is used as an inducing factor, so that the content of the neutral lipid in the microalgae cells can be increased quickly, production cost of biological energy resources is lowered, environment-friendly efficient production of bio-diesel raw materials is realized and little pollution is caused to water environment. Especially, when the oxidant H2O2 is used as the inducing factor, H2O2 can quickly degrade H2O and O2 in light and is free of pollution to water environment.

Description

technical field [0001] The invention belongs to the technical field of microalgae biology and bioenergy, and more particularly relates to a method for treating microalgae with an oxidant to efficiently induce rapid accumulation of neutral lipids, which can be used in the production of microalgae bioenergy. Background technique [0002] Microalgae are prokaryotic or eukaryotic unicellular photosynthetic microorganisms. They are very efficient solar energy converters. They are distributed in fresh or salt water and absorb light energy, water and CO transmitted by the water environment. 2 By accumulating biomass, light energy can be converted into chemical energy and stored in cells in the form of organic matter such as oil or starch. As the oldest lower photosynthetic organisms, some microalgae can directly utilize sunlight, CO 2 And simple nutrients such as N and P grow rapidly and synthesize a large amount of lipids (mainly neutral lipid triglycerides) in cells, thereby pro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/89
CPCY02E50/10
Inventor 王强陈辉张云明何晨柳
Owner INST OF AQUATIC LIFE ACAD SINICA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com