Method of utilizing oxidant to treat microalgae to efficiently induce neutral lipid for quick accumulation

A technology of oxidant and neutral lipid, which is applied in the fields of microalgae and bioenergy, can solve problems such as water pollution, and achieve the effects of improving productivity, high efficiency and productivity, and high induction efficiency

Inactive Publication Date: 2016-02-10
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The way of adding some special ingredients to induce due to the addition of some substances may cause water pollution to a certain extent

Method used

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  • Method of utilizing oxidant to treat microalgae to efficiently induce neutral lipid for quick accumulation
  • Method of utilizing oxidant to treat microalgae to efficiently induce neutral lipid for quick accumulation

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Embodiment 1

[0022] A method for efficiently inducing rapid accumulation of neutral lipids by treating microalgae with oxidants, the steps are (taking 1L culture scale as an example):

[0023] 1. Chlorella autotrophic culture:

[0024]The sterile BG11 liquid medium was added to six 2L sterile Erlenmeyer flasks respectively, and the added volume was 1L to prepare six BG11 liquid medium. The purified Chlorella sp.C2 (Chen, H., Zhang, Y.M., He, C.L., Wang, Q., 2014. Plant & Cell Physiology, 55: 634-644) was inoculated on six BG11 liquid medium, the seeding density is OD 700 =0.05 (about 1.1×10 6 cells / mL), and finally at the same time at a temperature of 25 °C and a light intensity of 70 μmolm -2 s -1 cultured under continuous aeration conditions.

[0025] 2. Oxidant-induced treatment

[0026] In this method, a standard curve between OD value and biomass is established with 700 nm as the scanning wavelength, and the concentration of biomass in the liquid medium is calculated by measurin...

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Abstract

The invention discloses a method of utilizing an oxidant to treat microalgae to efficiently induce neutral lipid for quick accumulation. The method includes: disposing the microalgae in an autotrophic culture medium for conventional autotrophic culture; when the microalgae are in the middle stage of logarithmic growth, starting to slowly adding a solution containing the oxidant H2O2, Bengal rose, peroxynitrite, decadienal or methyl viologen dropwise, and inducing accumulation of neutral lipid of microalgae cells, wherein content of the neutral lipid in the microalgae cells can be made equal to or higher than that of the neutral lipid generated by using other induction methods for treatment through induced culture for only 24-48h. The method is simple and easy to implement, and the oxidant is used as an inducing factor, so that the content of the neutral lipid in the microalgae cells can be increased quickly, production cost of biological energy resources is lowered, environment-friendly efficient production of bio-diesel raw materials is realized and little pollution is caused to water environment. Especially, when the oxidant H2O2 is used as the inducing factor, H2O2 can quickly degrade H2O and O2 in light and is free of pollution to water environment.

Description

technical field [0001] The invention belongs to the technical field of microalgae biology and bioenergy, and more particularly relates to a method for treating microalgae with an oxidant to efficiently induce rapid accumulation of neutral lipids, which can be used in the production of microalgae bioenergy. Background technique [0002] Microalgae are prokaryotic or eukaryotic unicellular photosynthetic microorganisms. They are very efficient solar energy converters. They are distributed in fresh or salt water and absorb light energy, water and CO transmitted by the water environment. 2 By accumulating biomass, light energy can be converted into chemical energy and stored in cells in the form of organic matter such as oil or starch. As the oldest lower photosynthetic organisms, some microalgae can directly utilize sunlight, CO 2 And simple nutrients such as N and P grow rapidly and synthesize a large amount of lipids (mainly neutral lipid triglycerides) in cells, thereby pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/89
CPCY02E50/10
Inventor 王强陈辉张云明何晨柳
Owner INST OF AQUATIC LIFE ACAD SINICA
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