SRAP (Sequence Related Amplified Polymorphism) molecular marker primer for identifying common cynodon dactylon and cynodon arcuatus as well as method and application of SRAP molecular marker primer

A technology of molecular markers and bermudagrass, which is applied in the field of DNA marker detection in molecular biology, can solve problems such as research reports, and achieve the effects of simple operation, clear bands, and good polymorphism

Pending Publication Date: 2016-03-23
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • SRAP (Sequence Related Amplified Polymorphism) molecular marker primer for identifying common cynodon dactylon and cynodon arcuatus as well as method and application of SRAP molecular marker primer
  • SRAP (Sequence Related Amplified Polymorphism) molecular marker primer for identifying common cynodon dactylon and cynodon arcuatus as well as method and application of SRAP molecular marker primer
  • SRAP (Sequence Related Amplified Polymorphism) molecular marker primer for identifying common cynodon dactylon and cynodon arcuatus as well as method and application of SRAP molecular marker primer

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Embodiment Construction

[0025] Referring to the accompanying drawings, the present invention will be further described in conjunction with specific embodiments, so as to better understand the present invention.

[0026] 1. Plant materials and reagents

[0027] 18 common bermudagrass and 28 curly bermudagrass: from the Institute of Tropical Crops Varieties and Resources, Chinese Academy of Tropical Sciences, Danzhou City, Hainan Province.

[0028] 1M / LTris-HCl (PH8.0) 200ml. 0.5M / LEDTA (PH8.0) 400ml. CTAB-free buffer solution 100ml: weigh 1.5gNaCl, add 50mlddH2O, stir and add 10mlEDTA (0.5M / L, pH8.0) and 20mlTris-HCl (1M / L, pH8.0) for sterilization, add 1ml β-mercaptoethanol and 2g PVP, dilute to 100ml with sterilized water. 3×CTAB buffer solution 100ml: weigh 3% CTAB (hexadecyltrimethylammonium bromide) (W / V) 3g and NaCl 8.8g and dissolve in 60ml water, then add 5ml EDTA (0.5M / L, PH8. 0) and 10ml Tris-HCl (1M / L, PH8.0) for sterilization, after sterilization, dilute to 100ml with sterilized water....

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Abstract

The invention discloses an SRAP (Sequence Related Amplified Polymorphism) molecular marker primer for identifying common cynodon dactylon and cynodon arcuatus. The SRAP molecular marker prime is selected from one of primer combinations of E1M4, E1M5, E1M6, E1M7, E1M8, E2M3, E2M4, E2M5, E2M6, E2M7, E3M1, E3M3, E3M4, E3M6, E3M9, E4M1, E4M3, E4M4, E4M5, E4M6, E5M9, E6M1, E6M2, E6M3, E6M4, E6M7, E6M8, E6M9, E7M2, E7M3, E7M4, E7M5, E7M7 and E10M9, wherein various primers are as shown in sequence. The SRAP molecular marker primer disclosed by the invention is used for identifying the cynodon richard; an obtained band is clear, good in polymorphism and high in repeatability; in addition, the SRAP molecular marker primer is simple in operation and wide in application prospect.

Description

technical field [0001] The invention relates to the technical field of DNA marker detection in molecular biology, in particular to an SRAP molecular marker primer for identifying common bermudagrass and bermudagrass and its method and application. Background technique [0002] Bermudagrass (CynodonRichard), also known as Bermuda grass, ground grass, salty sand grass, iron wire grass and tripping root grass, belongs to Poaceae (Poaceae / Gramineae) Chloridoideae (Cynodonteae) Bermudagrass genus (Cynodon Richard) C 4 Type perennial herb. It is the best rated warm-season turfgrass in the world, and it is also one of the most widely used turfgrasses. Of course, it is also a high-quality feed for livestock. There are currently 9 species and 10 varieties of Bermudagrass in the world, and there are 2 species and 1 variety in my country, which are Cynodondactylon and Cynodonarcuatus, and the variant is Cynodondactylon var. biflorus Merino). In my country, it is mainly distributed i...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156
Inventor 黄春琼刘国道白昌军
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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