A kind of detection method of dendrobium medicinal material

A detection method, the technology of Dendrobium, applied in the direction of measuring device, material separation, analysis of materials, etc., can solve the problems of non-repeatability, one-sided evaluation method, insufficient system, and complete indication of the overall quality of Dendrobium medicinal materials, and achieve good reproducibility , keep the effect intact, and the pre-processing method is simple

Active Publication Date: 2017-08-29
深圳市兰标科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a detection method for Dendrobium medicinal materials, to solve the problem that the quality evaluation of Dendrobium medicinal materials is mainly limited to the quality evaluation of individual or certain types of components. Technical issues indicating the overall quality of Dendrobium medicinal materials

Method used

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  • A kind of detection method of dendrobium medicinal material
  • A kind of detection method of dendrobium medicinal material
  • A kind of detection method of dendrobium medicinal material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: Dendrobium candidum detection method

[0047] Instruments and reagents: Instruments: Agilent1200series high performance liquid chromatography (including low-pressure binary gradient pump G1312A, column thermostat G1316A, diode array detector G1315B, Chemstation chemical workstation, American Agilent Technology Co., Ltd.), 100g portable pulverizer (Guangzhou Xulang Machinery Equipment Co., Ltd.) Milli-Q Synthesis ultrapure water purification system (Millipore Company), RQ-250B ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.), PB303-N electronic balance (MEETTLER TOLEDO, 0.001g), 0.45μm microporous filter head (Jinteng Company). Reagents: acetonitrile, methanol (chromatographically pure, Fisher Scientific, USA); other reagents are analytically pure; ultrapure water (MilliQ ultrapure water, passed through a 0.45 μm microporous membrane); acetic acid (Beijing Dongfang Fine Chemicals Co., Ltd.).

[0048] The chromatographic conditions for the estab...

Embodiment 2

[0062] Embodiment two: the detection method of Dendrobium chrysoprase

[0063] The difference from Example 1 is:

[0064] Preparation of standard sample solution: take different batches of dry samples of Dendrobium chrysalis, pulverize them with a pulverizer, pass through a pharmacopoeia sieve (aperture 0.335mm), accurately weigh 1.000g of Dendrobium powder, place it in a 100ml Erlenmeyer flask, add 50mL 75 % methanol (V water: V methanol = 25:75), take it out after ultrasonication for 30 minutes at room temperature, filter, and concentrate the filtrate to dryness by rotary evaporation, dissolve it with 75% methanol solvent (V water: V methanol = 25:75), and finally transfer Dilute to a 5ml volumetric flask, shake well, and filter with a 0.45μm microporous membrane to obtain standard sample solutions of different batches.

[0065] Table 3 Similarity evaluation results of 10 batches of Dendrobium drumstick fingerprints

[0066]

[0067] According to the method described ab...

Embodiment 3

[0069] Embodiment three: the detection method of Dendrobium dendrobii

[0070] The difference from Example 1 is:

[0071] Preparation of standard sample solution: take different batches of dry samples of Dendrobium nodii, pulverize them with a pulverizer, pass through a pharmacopoeia sieve (aperture 0.335mm), accurately weigh 1.000g of Dendrobium powder, place it in a 100ml Erlenmeyer flask, add 50mL 75 % methanol (V water: V methanol = 25:75), take it out after ultrasonication for 30 minutes at room temperature, filter, and concentrate the filtrate to dryness by rotary evaporation, dissolve it with 75% methanol solvent (V water: V methanol = 25:75), and finally transfer Dilute to a 5ml volumetric flask, shake well, and filter with a 0.45μm microporous membrane to obtain standard sample solutions of different batches.

[0072] Table 4 Similarity evaluation results of fingerprints of 10 batches of Dendrobium dendrobii

[0073]

[0074] According to the above method, 10 bat...

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Abstract

The invention relates to a quality evaluation method for a dendrobe medicinal material. The method includes the steps that a schaftoside chromatographic peak is adopted as a reference substance, and a standard fingerprint spectrum is formulated through HPLC (high performance liquid chromatography); through comparison of fingerprint spectrums of samples for test and the standard fingerprint spectrum, the quality of the samples for test is determined. According to the method, the pretreatment method for all the samples for test is simple, characteristic components are reserved, and the solution stability of the samples for test is good. The high performance liquid chromatography is high in precision, good in reproducibility and short in analysis time, and has certain specificity, the separation effect of all fingerprint peaks in the obtained fingerprint spectrum is good, the quality of the dendrobe medicinal material can be accurately and comprehensively reflected, the quality standard of the dendrobe medicinal material is further perfected, and therefore the quality evaluation method can be applied to quality grading and authenticity of dendrobe tissue culture seedlings, fresh dendrobe and air-dried dendrobe of medicinal dendrobe.

Description

technical field [0001] The invention relates to the quality analysis of medicinal materials in the field of traditional Chinese medicines, in particular to a detection method for various dendrobium medicinal materials by HPLC (high performance liquid chromatography) fingerprinting. Background technique [0002] Dendrobium is a commonly used tonic Chinese medicine, mainly for the Dendrobium genus. Dendrobium is the largest genus of Orchidaceae, including Dendrobium candidum, Dendrobium nobile, Dendrobium dendrobii, Dendrobium drumstick and other varieties. There are more than 1,100 species of Dendrobium in the world, of which 76 are found in my country. Dendrobium has a long history of medicinal value. As early as in ancient times, Dendrobium was listed as a nourishing top grade in "Shen Nong's Materia Medica". For a long time, with the development of the times, Dendrobium has been regarded as a precious Chinese herbal medicine with very important nourishing effects. Dendr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 梁琼麟邵自星梁晓萍赵田
Owner 深圳市兰标科技有限公司
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