Production method for tagatose

一种生产方法、塔格糖的技术，应用在差向异构生产塔格糖领域，能够解决未能公开塔格糖等问题，达到降低生产成本、高产率的效果

Active Publication Date: 2016-03-23
CJ CHEILJEDANG CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, both documents fail to disclose a method for the production of tagatose by C4-epimerization of fructose

Method used

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  • Production method for tagatose

Examples

Experimental program
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Effect test

example 1

[0060] Evaluation of Chromatographic Separation Characteristics Based on Enzyme Conversion

[0061] In order to separate and crystallize a target material (tagatose) with high purity from mixed sugars (fructose and tagatose), purification using chromatographic separation is extremely important. In general, different recovery yields (%) are achieved for purified sugar solutions to obtain crystallized products, depending on the content of the target material (tagatose). High recovery yields (%) are obtained proportionally with increasing content of target material (tagatose). Thus, it is possible to produce the final product with relatively low production and processing costs. In order to efficiently produce crystalline sugar (high purity, high yield), it is extremely important to ensure that the purity of the target material (tagatose) contained in the feed (Feed) is 90% or more, more preferably 95%.

[0062] The operating conditions of chromatography can produce various resu...

example 2

[0074] Preparation of Ketohexose C4-epimerase

[0075] Genomic DNA (SEQ ID NO. 1) of Thermotoga maritima (strain ATCC43589 / MSB8 / DSM3109 / JCM10099) was used as a template, a thermotolerant microorganism and forward primers (5'-GGGCATATGATGGTCTTGAAAGTGTTCAAAG-3') and reverse Primer (5'-AAACTCGAGCCCCTCCAGCAGATCCACGTG-3') was used for PCR.

[0076] In addition, by using genomic DNA (SEQ ID NO: 2) of Thermotoga neoapollinii (DSM4359) as a template, a heat-resistant microorganism and adding a forward primer (5'-GGGCATATGATGGTCTTGAAAGTGTTCAAAG-3') and a reverse primer ( 5'-AAACTCGAGTCACCCCCTTCAACAGGTCTACGTG-3') for PCR.

[0077] Conditions of PCR are shown in Table 3 and Table 4. The gene amplified by PCR was inserted into pET-21a vector, followed by transformation into E. coli DH5α strain. Plasmids were isolated from transformed strains, which were sequenced to identify the base sequence of the inserted gene. The plasmid was transformed into the protein expression strain Escheric...

example 3

[0085] Purification of Ketohexose C4-epimerase

[0086] In order to measure the activity of ketohexose C4-epimerase expressed in microorganisms, protein purification was performed by the following method. The culture solution that completed the protein expression was centrifuged at 8,000rpm for 10 minutes to collect the microorganisms, and the microorganisms were resuspended in 50mM NaH containing 10mM imidazole (imidazole) and 300mMNaCl 2 PO 4 (pH8.0) buffer. Suspended microorganisms were disrupted by a sonicator and centrifuged at 13,000 rpm for 10 minutes to collect a supernatant. The collected supernatant was passed through a chromatography column packed with Ni-NTA resin as a filler. At this point, 50 mM NaH containing 20 mM imidazole and 300 mM NaCl 2 PO 4 (pH8.0) buffer flowed through in an amount of 10 times the volume of the filler in the chromatographic column, thereby removing proteins non-specifically attached to the filler. Finally, 50 mM NaH containing 250 ...

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Abstract

The present invention relates to a production method for tagatose.

Description

technical field [0001] The present invention relates to a method for the production of tagatose from fructose, more precisely, to a method for the production of tagatose by epimerization of fructose at the carbon 4 position. Background technique [0002] Tagatose has a natural sweetness that is almost indistinguishable from sucrose and has similar physical properties to sucrose. However, ingested tagatose is not well absorbed in the small intestine and therefore has minimal effect on blood sugar levels. In addition, tagatose is a low-calorie sweetener with about 30% of the calories of sucrose. In addition, tagatose has a prebiotic effect of promoting the growth of beneficial lactic acid bacteria through fermentation by gut microflora. [0003] Only about 20% of ingested tagatose is absorbed in the small intestine, and the remaining 80% part of the tagatose reaches the large intestine where the intestinal microflora grows and selectively promotes the production of lactic ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/24C12P19/02
CPCC12P19/02C12P19/24Y02P20/582C12N9/90C12Y501/03C07C45/81C12P7/26
Inventor 梁成才金良姬金成俌朴承源朴逸香金敏会李英美
Owner CJ CHEILJEDANG CORP
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