Molecular identification method of clerodendranthus spicatus

Abstract

The invention belongs to the field of biotechnology, and in particular relates to a molecular identification method of clerodendranthus spicatus. The molecular identification method disclosed by the invention, by screening an SRAP (sequence-related amplified polymorphism) primer combination, can enhance the capacity of identifying the clerodendranthus spicatus of approximate varieties, and through amplification and comparison on the approximate varieties, primers can effectively distinguish the various clerodendranthus spicatus varieties and can conduct molecular identification on the clerodendranthus spicatus varieties. Meanwhile, the method disclosed by the invention, through the direct identification of genetic materials of the clerodendranthus spicatus, can greatly improve the accuracy of identification. The molecular identification method disclosed by the invention can conduct the identification of the clerodendranthus spicatus varieties on a DNA level and can avoid errors caused by such indirect identification methods as expression identification; the method has the characteristics of convenient operation and good repeatability; and the method is high in reliability and authority.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular identification method of purple flower kidney tea. Background technique [0002] Kidney tea (Clerodendranthus spicatus) is a perennial herb of the Lamiaceae Kidney tea genus. Because of its slender stamens, it resembles a cat's whiskers, so it has another name called cat whiskers. Kidney tea is a very commonly used herbal medicine in Southeast Asia. It has been introduced in my country very early and is currently distributed in Fujian, Hong Kong, Hainan, Guangdong, Taiwan, Yunnan, Guangxi, Sichuan and other places. [0003] Kidney tea has obvious medicinal and health care functions. Kidney tea has medical and health care functions such as diuresis, stone removal, antibacterial, anti-inflammatory, kidney strengthening, improvement of chronic renal failure and immunity. Its extract has already been used as a health product for the treatment of chronic nephritis...

AI Technical Summary

Problems solved by technology

Although it is simple and intuitive to judge based on morphological characteristics, the growth and development of kidney tea are easily affected by changes in external environmental conditions, and the overall morphological characteristics of the plant are unstable. In addition, it cannot be judged from the color of petals when it is in the seedling stage or non-flowering stage. distinguish

In short, the traditional identification methods (character, microstructure and chemical identification methods) are easily interfered by many factors such as the growth environment, growth stage, and processing of medicinal materials.

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