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Avian tuberculosis hyperimmune serum and preparation method thereof

A high-immunity serum, avian tuberculosis technology, applied in the biological field, can solve the problems of long immunization time, high cost, complicated emulsification operation, etc., and achieve the effect of good effect, low cost and low condition requirements.

Inactive Publication Date: 2020-09-01
SICHUAN AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method is costly, the emulsification operation is more complicated, and the time required for immunization is longer

Method used

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  • Avian tuberculosis hyperimmune serum and preparation method thereof
  • Avian tuberculosis hyperimmune serum and preparation method thereof
  • Avian tuberculosis hyperimmune serum and preparation method thereof

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Experimental program
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Effect test

Embodiment

[0037] The preparation method of poultry tuberculosis hyperimmune serum of the present invention is as follows:

[0038] A. Preparation of immune antigen

[0039] a) The avian Mycobacterium tuberculosis cultured in 30ml of liquid culture medium is subdivided into 50ml centrifuge tubes, centrifuged at 8000g for 15min, and the supernatant is gently sucked up with a Pasteur pipette and discarded;

[0040] b) Add 30ml of sterilized PBS buffer solution, centrifuge at 9000g for 15min, gently absorb the supernatant with a Pasteur pipette and discard it;

[0041] c) Add 30ml of sterilized PBS buffer solution, centrifuge at 10000g for 15min, gently absorb the supernatant with a Pasteur pipette and discard it;

[0042] d) Add 15ml of formaldehyde solution with a mass fraction of 0.3%, place in a 37°C incubator for 24 hours, and then place at room temperature for 24 hours; centrifuge at 10,000g for 15 minutes, gently absorb the supernatant with a Pasteur pipette and discard it;

[0043...

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Abstract

The invention discloses avain tuberculosis hyper-immune serum and a preparation method thereof. The preparation method includes the steps that inactivated avain tuberculosis bacilli are directly adopted as immunizing antigens, immunizing is conducted with an ear edge intravenous injection method, immunizing is conducted once every three days, immunizing is conducted seven times in total, blood sampling is conducted seven days after the last time of immunizing, and serum is collected. The high-potency serum can be prepared only through the inactivated bacilli via intravenous injection on immune animals, emulsifying operation is not needed, the whole hyper-immune serum preparation process only lasts for 26 days, the antibody valence measured by a tube agglutination test reaches 1 / 128, and compared with a conventional hyper-immune serum preparation method, the requirements for preparation conditions for the immunizing antigens involved in the method are low, immunizing procedure operation is simpler, needed time is shorter, and the high-potency antibody level can be acquired.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a poultry tuberculosis hyperimmune serum and a preparation method thereof. Background technique [0002] Avian tuberculosis (Avian tuberculosis) is a chronic contact infectious disease caused by Mycobacterium avian tuberculosis. It is mainly infected through the respiratory tract and digestive tract. Infected poultry mainly manifests as rough coat, decreased egg production, emaciated body, and prominent breastbone. It is as thin as a blade, and even eventually leads to death. Granulomas and calcified nodules of various sizes are formed in tissues and organs. [0003] The cell wall of Mycobacterium avium tuberculosis contains a large amount of lipids and has strong resistance to external factors. Isolation and cultivation of Mycobacterium avian tuberculosis requires a special medium. On a medium containing whole egg liquid or egg yolk liquid, culture at 37.5°C-40°C for 10...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/12C07K16/06
CPCC07K16/06C07K16/1289
Inventor 朱德康陈泓汐程安春汪铭书宋晓恒
Owner SICHUAN AGRI UNIV
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