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Early-period treatment method of jellyfish podocyst paraffin section

A technology of paraffin sectioning and pre-processing, applied in the preparation of test samples, etc., can solve the problems of inconvenient operation, difficult to fix, small size, etc., and achieve the effect of good effect, strong practicability and simple operation.

Inactive Publication Date: 2016-04-20
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the defects that the jellyfish foot capsule is difficult to peel off from the attachment base, difficult to fix, small in size and inconvenient to operate, and provide a pre-treatment method for the paraffin section of the jellyfish foot capsule, which can realize the complete fixation of the foot capsule , and can locate the position of the foot capsule

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Sampling treatment: Rinse the polyethylene corrugated board with jellyfish foot sacs with seawater, select the part with more foot sacs, and cut the corrugated board with foot sacs into small pieces with a length and width of 1.5 cm.

[0020] 2. The first fixation: place the corrugated plate with the foot capsule in the above step 1 in a 50ml centrifuge tube, add Bouin's fixative solution and fix it for 60min.

[0021] 3. Foot capsule puncture: gently wash the foot capsule fixed in the above step 2 with phosphate buffer solution for 5 times, place it under a stereoscope, and use a fine needle to cut the chitin outside the side of the foot capsule obliquely downward. membrane piercing.

[0022] 4. The second fixation: put the foot capsule treated in the above step 3 into Bouin's fixative solution again and fix it for 1 hour.

[0023] 5. Collection of foot capsules: Wash the foot capsules treated in the above step 4 twice with phosphate buffer, place them under a ster...

Embodiment 2

[0028] 1. Sampling treatment: Rinse the polyethylene corrugated board with jellyfish foot sacs with seawater, select the part with more foot sacs, and cut the corrugated board with foot sacs into small pieces with a length and width of 1 cm.

[0029] 2. The first fixation: Place the corrugated plate with the foot capsule in step 1 above in a 50ml centrifuge tube, add Bouin's fixative solution and fix it for 30min.

[0030] 3. Foot capsule puncture: gently wash the foot capsule fixed in the above step 2 with phosphate buffer three times, place it under a stereoscope, and use a fine needle to cut the chitin outside the side of the foot capsule obliquely downward. membrane piercing.

[0031] 4. The second fixation: put the foot capsule treated in the above step 3 into Bouin's fixative solution again and fix it for 2 hours.

[0032] 5. Collection of foot capsules: Wash the foot capsules treated in the above step 4 with phosphate buffer for 3 times, place them under a stereoscope,...

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PUM

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Abstract

The invention provides an early-period treatment method of a jellyfish podocyst paraffin section and belongs to the technical field of preparation of coelenterate tissue sections. The early-period treatment method comprises the following steps: carrying out sampling treatment, fixing for the first time, puncturing a podocyst, fixing for the second time, collecting the podocyst, carrying out ultrasonic washing, agglutinating agar and fixing for the third time. Under the condition of guaranteeing the integrity of tissue forms, the jellyfish podocyst is completely fixed; the method has high practicability, is simple to operate and has a good podocyst fixing effect; the development of subsequent researches of manufacturing of the jellyfish podocyst paraffin section and the like is facilitated.

Description

technical field [0001] The invention belongs to the technical field of preparation of coelenterate tissue slices, and in particular relates to a pre-treatment method for jellyfish paw sac paraffin slices. Background technique [0002] Jellyfish is a large edible jellyfish endemic to the coastal areas of my country and one of the important species in aquaculture in China. The research on its reproductive biology has always been valued by relevant researchers. The podocystis is a key stage of the jellyfish's asexual reproduction life cycle. It can withstand harsh environments such as low oxygen and lack of bait, and can prevent predators from predators and harmful microorganisms. It is of great significance for maintaining natural resources of jellyfish and realizing artificial breeding. [0003] Paraffin section technique is widely used in the observation of histological morphology, which is of great significance in the study of jellyfish foot capsule. The jellyfish foot cap...

Claims

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Application Information

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IPC IPC(8): G01N1/30G01N1/36
CPCG01N1/30G01N1/36
Inventor 孙云刘春胜陈四清顾志峰王爱民陈昭廷
Owner HAINAN UNIVERSITY
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