Cerebrospinal fluid and urinary protein liquid single reagent and preparation method thereof
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A cerebrospinal fluid, single-reagent technology, applied in material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc. problem, to achieve the effect of improving sensitivity, darkening color, and good repeatability
Active Publication Date: 2016-05-04
JILIN GETEIN BIOTECH CO LTD
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The change of its turbidity, the chemical properties of the acidic precipitant, the temperature and the concentration of the acid all affect the determination, and the anti-interference performance is poor. It is generally used for qualitative and rarely for quantitative
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Embodiment 1
[0019] Weigh 5.94g of succinic acid and add it to 1L of purified water, stir to dissolve, then add 0.5g of sodium benzoate, stir to dissolve, and adjust the pH to 2.0 (25°C) with HCl as the succinic acid-sodium benzoate buffer for later use. Measure TritonX-3055ml into 20ml dimethyl sulfoxide, stir and mix well, then add 0.01g of pyrogallol red, stir until completely dissolved, add to succinic acid-sodium benzoate buffer, then add 0.012 g sodium molybdate, 1.39g sodium oxalate, 0.09g 2-formyl-1,1-dimethylhydrazine.
Embodiment 2
[0021] Weigh 5.94g of succinic acid and add it to 1L of purified water, stir to dissolve, then add 0.5g of sodium benzoate, stir to dissolve, and adjust the pH to 2.6 (25°C) with HCl as the succinic acid-sodium benzoate buffer for later use. Measure 10ml of TritonX-305 into 50ml of dimethyl sulfoxide, stir and mix well, then add 0.05g of pyrogallol red, stir until completely dissolved, add to succinic acid-sodium benzoate buffer, then add 0.024 g sodium molybdate, 2.78g sodium oxalate, 0.27g 2-formyl-1,1-dimethylhydrazine.
Embodiment 3
[0023] Weigh 5.94g of succinic acid and add it to 1L of purified water, stir to dissolve, then add 0.5g of sodium benzoate, stir to dissolve, and adjust the pH to 3.5 (25°C) with HCl as the succinic acid-sodium benzoate buffer for later use. Measure 20ml of TritonX-305 into 100ml of dimethyl sulfoxide, stir and mix well, then add 0.1g of pyrogallol red, stir until completely dissolved, add to succinic acid-sodium benzoate buffer, and then add 0.0484 g sodium molybdate, 5.56g sodium oxalate, 0.54g 2-formyl-1,1-dimethylhydrazine.
[0024] Prove positive effect place of the present invention by following comparative example:
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Abstract
The invention provides a cerebrospinal fluid and urinary protein liquid single reagent detection reagent kit and a preparation method thereof and belongs to field of in-vitro diagnosis reagents in order to solve the problems that an existing cerebrospinal fluid and urinary protein liquid single reagent detection reagent kit is low in precision and stability, and reagents contaminate instruments. The reagent kit is composed of a single reagent. The invention further provides the preparation method of the cerebrospinal fluid and urinary protein liquid single reagent detection reagent kit. In the reagent, dimethyl sulfoxide is used for dissolving pyrogallol red, TritonX-305 is added into dimethylsulfoxide before dissolving, then pyrogallol red is added, reagent adhesive cuvette can be effectively removed, and the cuvette is prevented from being contaminated. Besides, pyrogallol red is dispersed evenly, and sensitivity and precision of the reagent are improved. 2-formyl-1,1-dimethylhydrazine is added into the reagent, so that a bluish violet composition formed by pyrogallol red-molybdate and protein obviously becomes darker, and reagent sensitivity is improved.
Description
technical field [0001] The invention provides a single reagent for cerebrospinal fluid and urine protein liquid and a preparation method thereof, belonging to the field of in vitro diagnostic reagents. Background technique [0002] At present, the commonly used analytical methods of cerebrospinal fluid and urine protein in the market include pyrogallol red molybdenum method, biuret method and sulfosalicylic acid turbidimetric method. Biuret is a compound formed by heating and condensing two molecules of urea. Biuret forms a purple-red complex with copper sulfate under alkaline conditions. The protein molecule contains peptide bonds, which are similar in structure to biuret. Therefore, proteins and Basic copper sulfate can also form a purple-red complex, and its color depth is proportional to the protein concentration under certain conditions, which can be used for protein quantification. However, no matter the manual method or the kit, there are problems such as high measur...
Claims
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