Preprocessing method and device for image recognition of DNA sequence

A DNA sequencing and image recognition technology, applied in the field of DNA sequencing analysis, can solve problems affecting base types, difficult identification of targets and backgrounds, and misidentification of targets

Active Publication Date: 2016-05-04
BEIJING ZHONGKEZIXIN TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the image acquisition of the reaction chip, the target and the background are not easy to identify, which will cause misidentification of the target, which will affect the accuracy of image recognition and seriously affect the judgment of the base type.

Method used

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  • Preprocessing method and device for image recognition of DNA sequence
  • Preprocessing method and device for image recognition of DNA sequence
  • Preprocessing method and device for image recognition of DNA sequence

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0066] see figure 1 As shown, it is a flow chart of the image recognition preprocessing method for DNA sequencing provided by Embodiment 1 of the present invention, including:

[0067] Step 101, obtain the spectrum information, and obtain the DNA spectrum within the sampling time interval of each spectrum;

[0068] Specifically, the acquisition of the DNA map can be achieved in the following ways:

[0069] The reaction solution enters the reaction chip of the DNA sequencer for a chemical reaction to generate visible light; the CCD camera takes pictures of the visible light information generated in the reaction chip at the appropriate photographing position to collect the DNA spectrum.

[0070] More specifically, when obtaining the map information, for the signal waveform, in each continuous K cycle, select n sampling points at preset moments in each cycle, and each interval time T 0 Sampling once, continuous sampling M times; in order to ensure the referenceability and accur...

Embodiment 2

[0088] figure 2 For the flow chart of the image recognition preprocessing method for DNA sequencing provided in Embodiment 2 of the present invention, please refer to figure 2 On the basis of Example 1, the image recognition method for DNA sequencing provided in this example includes:

[0089] Step 201, acquiring four fluorescent images of CY3, CY5, FAM and TXR;

[0090] Specifically, when the DNA spectrum captured by the CCD camera is blurred, but the fluorescence image is clear, the magnetic bead identification can be performed by acquiring the fluorescence image.

[0091] Step 202, using the atlas as a reference image, registering the four fluorescence images to obtain a registered fluorescence image;

[0092] Step 203, finding the center point of the magnetic beads in the registered fluorescent image;

[0093] Step 204, performing fusion processing on the central point to obtain the fused central point of the magnetic bead.

[0094] Specifically, in general, one of t...

Embodiment 3

[0097] image 3 For the flow chart of the image recognition preprocessing method for DNA sequencing provided in Example 3 of the present invention, please refer to image 3 , this embodiment is further limited on the basis of embodiment two, in this embodiment,

[0098] The center point of the magnetic bead searched in the registered fluorescent image specifically includes:

[0099] Step 301, identify the magnetic bead pixels on the spectrum, if the absolute value of the minimum value of f(i,j) min(i,j) difference is greater than or equal to T 0 , it is identified as a magnetic bead, otherwise, it is a background pixel.

[0100] Preferably, it can also include:

[0101] Step 302, traversing the pixels of the magnetic bead to obtain the central pixel of the magnetic bead, wherein the central pixel of the magnetic bead is, the gray value of the current point is equal to the maximum gray value in the 4*4 pixel area centered on the current point, and The four neighbors of the ...

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Abstract

The invention relates to a preprocessing method and device for the image recognition of a DNA sequence, and the method comprises the steps: obtaining map information, and respectively obtaining a DNA map in a sampling time interval of each map; carrying out the gray scale linear stretching of the maps, and obtaining stretched DNA maps; obtaining a first pixel and a second pixel of each DNA map after stretching; calculating a global threshold T of gray scale mean values of the first and second pixels; calculating the variance sigma2 of the first and second pixels; and carrying out the segmentation of the maps through employing the global threshold T if the variance sigma2 is within a preset range. The method can achieve purposes of enabling a target and background to be easy to recognize and avoiding the wrong recognition of the target during the collection of an image of a reaction chip. Moreover, the method is short in operation time, is good in image segmentation effect, improves the accuracy of image recognition after the collection of the image of the reaction chip, and precisely determines the type of a basic group.

Description

technical field [0001] The invention relates to the field of DNA sequencing analysis, in particular to a preprocessing method and device for image recognition of DNA sequencing. Background technique [0002] In the field of DNA sequencing technology, the overall operation process is described as follows: After the DNA sample is crushed, the library building reagent is used to add adapters, single-strand capture, bind to microspheres, microemulsion PCR amplification, and emulsion breaking, and obtain the microspheres. For the DNA library, the library and the enzymes required for the sequencing reaction are placed on the sequencing chip with a micro-reaction pool by using the sample plate, and the sequencing chip and sequencing reagents are installed on the host computer, and the sequencing program is started by the control computer according to the number and position of the modules, which is automated The sequencing reaction is carried out, and the generated data is transmit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06T7/00
CPCG06T7/0012G06T2207/30072
Inventor 刘元杰陈哲张睿范东雨高科王者馥王绪敏殷金龙任鲁风
Owner BEIJING ZHONGKEZIXIN TECH
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