Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

In-vitro Nipah virus detection reagent and in-vitro Nipah virus detection method

A Nipah virus, in vitro detection technology, applied in microorganism-based methods, biochemical equipment and methods, and microbial determination/inspection, etc., can solve the problems of high biological hazard and high fatality rate, and achieve the effect of easy operation.

Inactive Publication Date: 2016-05-11
浙江国际旅行卫生保健中心
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fruit bats infected with Nipah virus have no obvious diseases. Once infected with humans, the fatality rate is extremely high and the biological hazard is extremely high. Currently, there is no vaccine or specific drug for the treatment of Nipah virus infection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • In-vitro Nipah virus detection reagent and in-vitro Nipah virus detection method
  • In-vitro Nipah virus detection reagent and in-vitro Nipah virus detection method
  • In-vitro Nipah virus detection reagent and in-vitro Nipah virus detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The detection of embodiment 1 Nipah virus

[0022] 1. Instruments and Materials

[0023] 7500 fluorescent quantitative PCR instrument and automatic nucleic acid extraction instrument were purchased from Life Company. Primers, probes and plasmids were synthesized by Beijing Zhongmei Taihe Biotechnology Co., Ltd., 10×Buffer, 2.5mMdNTP, Taq enzyme, and AMV enzyme were purchased from Beijing Meilaibo Medical Co., Ltd., and 1836 nucleic acid extraction kit was purchased from Life Company.

[0024] Primer and Probe Design

[0025] According to the sequences of 48 Nipah virus strains in the genbank database, select the conserved sequence of the main pathogenic protein of Nipah virus—G protein gene as the target gene, and design a pair of specific primers (NIF and NIR) and a probe (NIP), primer and probe sequences are as follows:

[0026] Forward primer NIF: 5'-GCAAGAGAGTAATGTTCAGGCTAGAG-3'

[0027] Reverse primer NIR: 5'-CTGTTCTATAGGTTCTTCCCCTTCAT-3'

[0028] Probe NIP: 5...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an in-vitro Nipah virus detection reagent. The in-vitro Nipah virus detection reagent comprises specific primers and a probe. A sequence of a forward primer NIF is shown as 5'-GCAAGAGAGTAATGTTCAGGCTAGAG-3'; a sequence of a reverse primer NIR is shown as 5'-CTGTTCTATAGGTTCTTCCCCTTCAT-3'; a sequence of the probe NIP is shown as 5'FAM-TGCAGGAGGTGTGCTCATTGGAGG-TAMRA3'. The invention further provides an in-vitro fluorescent PCR (polymerase chain reaction) Nipah virus detection method which adopts the in-vitro Nipah virus detection reagent to quickly and accurately detect Nipah viruses. The in-vitro fluorescent PCR Nipah virus detection method has the advantages of simplicity and convenience in operation, high efficiency, quickness and specificity; due to establishment of the method, sample detection can be finished only by about one hour, and a gap of domestic Nipah virus detection is filled.

Description

technical field [0001] The invention relates to a reagent and method for detecting Nipah virus in vitro. technical background [0002] Nipah virus is an emerging zoonotic virus (a virus that can be transmitted from animals to humans). Nipah virus causes severe illness in infected people, characterized by inflammation of the brain (encephalitis) or respiratory disease. The virus can also cause severe disease in animals such as pigs, causing significant economic losses to farmers. Nipah virus is an RNA virus belonging to the genus Hendra Nipahvirus, a new virus class in the Paramyxoviridae family. Fruit bats of the family Pteroptera are the natural hosts of Nipah virus. Fruit bats infected with Nipah virus have no obvious diseases. Once infected with humans, the fatality rate is extremely high and the biological hazard is extremely high. Currently, there is no vaccine or specific drug for the treatment of Nipah virus infection. [0003] The fluorescent PCR detection method...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
CPCC12Q1/701C12Q1/686C12Q2563/107
Inventor 吕沁风黄建可蔡玉峰吴忠华
Owner 浙江国际旅行卫生保健中心
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com