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Preparation method of urine HCG quantitative detection test paper strip

A quantitative detection and test strip technology, applied in the field of immunodiagnosis, can solve the problems of inability to quantify, difficult to conceive, quantify, etc., and achieve the effects of improved accuracy, low detection cost, and convenient sampling

Inactive Publication Date: 2016-05-11
SHANGHAI UPPER BIO TECH PHARMA
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Problems solved by technology

Most of the above methods are realized by coating the quality control line C with goat anti-mouse IgG. The concentration of the sample can only be quantified within a certain concentration range, and the range is too wide, and there is no quantification of the concentration above the HOOK effect. , so it cannot be quantified for samples greater than 15000mIU / ml, so it is difficult to carry out detailed monitoring of pregnancy

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  • Preparation method of urine HCG quantitative detection test paper strip
  • Preparation method of urine HCG quantitative detection test paper strip

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Embodiment Construction

[0029] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further described below in conjunction with specific embodiments.

[0030] see figure 1 , the preparation method of a kind of urine HCG quantitative detection test strip of the present invention, comprises the steps:

[0031] 1) Scratch film

[0032] Paste the nitrocellulose membrane on the PVC bottom plate with back glue, and then draw the detection line T solution and the quality control line C solution on the nitrocellulose membrane at the same time with a scriber, and dry it in a constant temperature oven at 37 ° C for 12- 24 hours; the final concentration of HCG antibody in the T-line solution is 1-2 mg / ml, the final concentration of antigen in the C-line solution is 1-2 mg / ml, and the spray volume of the T-line solution and C-line solution is 0.5-1 μl / cm.

[0033] 2) Handle the binding pad

[0034] Afte...

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Abstract

The invention discloses a preparation method of a colloidal gold immunity lateral chromatography test paper strip for detecting HCG concentration in a urine sample. A HCG antigen serves as a material of a quality control line C, the HCG antigen on the quality control line C and a detecting line T react, and a gold-labelled antibody is left. Along with increase of HCG concentration in the sample, a typical HOOK effect happens to the detecting line T, and within the concentration range where the HOOK effect happens, the color of a quality control line C strip becomes lighter. By means of color changes of a T line strip and the C line strip, quantitative detection can be performed on HCG urine samples within the concentration range of 0-200000 mIU / mL, the false negative phenomenon caused by the HOOK effect is avoided, and clinical detection accuracy is improved.

Description

technical field [0001] The invention relates to the field of immunodiagnosis, in particular to a preparation method of a urine HCG quantitative detection test strip. Background technique [0002] Human chorionic gonadotropin (HCG) is a glycoprotein hormone secreted by placental chorionic cells after fertilized eggs are implanted in women, and can enter blood and urine through blood circulation. HCG begins to be secreted on the 6th day after fertilization, and can be detected in the serum and urine of pregnant women on the 7th day after fertilization, which can be used for the diagnosis of early pregnancy. The serum concentration reached a peak at 8-10 weeks of pregnancy, about 50000-20000mIU / L, continued for 10 days and then decreased rapidly until delivery. Therefore, according to the concentration of HCG in urine or serum, an auxiliary diagnosis can be made on whether the pregnancy is normal or not. [0003] When the content of the antigen to be detected in the sample is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/76
CPCG01N33/76
Inventor 于灵芝杨晶李福刚袁晓航沈旭辉韩思超
Owner SHANGHAI UPPER BIO TECH PHARMA
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