A method for assisted high-efficiency breeding of maize using ssr and snp molecular markers in combination

A molecular marker, corn technology, applied in the fields of molecular biology and genetic breeding, can solve the problems of difficult to achieve high-throughput sites and high detection costs
CN105603083BActive Publication Date: 2019-12-17BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Publication Date
2019-12-17

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Abstract

The invention relates to a method of assistant efficient breeding of corns cooperatively using two molecular markers of SSR and SNP. The corn selfing line Jing SD127 is adopted as a donor parent while Jing SD121 is adopted as a recurrent parent; a BC1F1-generation group is obtained through backcross transformation; 6 SSR markers and 104 SNP markers are adopted for background selection for the BC1F1-generation group; and single plants with high background recovery rate are screened to increase the improvement speed of the corn selfing line Jing SD121. According to comparison of the background selection results of the SSR and SNP markers, the two markers are correlated in a middle degree. Therefore, the background selection can be performed by combining the SSR and SNP markers in the backcross early generation so as to realize large-group strict selection and improve the selection efficiency.
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Description

technical field

[0001] The invention relates to the fields of molecular biology and genetic breeding, in particular to a method for cooperating two molecular markers of SSR and SNP to carry out assisted high-efficiency breeding of maize. Background technique

[0002] Conventional maize breeding selects target individuals through phenotypic observation, which not only relies on the experience of breeders, but also is often affected by the interaction between genotype and environment, making it difficult to select the ideal genotype. Molecular marker-assisted selection is an auxiliary means of applying molecular markers in the process of crop variety improvement, mainly including foreground selection for target traits and background selection for genetic background. Using molecular markers to assist background selection in the process of backcross breeding can significantly speed up the speed of background recovery and shorten the breeding time.

[0003] Molecular markers sui...

Claims

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