A method for assisted high-efficiency breeding of maize using ssr and snp molecular markers in combination

A molecular marker, corn technology, applied in the fields of molecular biology and genetic breeding, can solve the problems of difficult to achieve high-throughput sites and high detection costs

Active Publication Date: 2019-12-17
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Description
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Problems solved by technology

PCR-based SSR markers have the advantages of high polymorphism, simple operation, and good repeatability, but it is difficult to achieve high throughput of sites
Sequence-based SNP markers have high distribution density, good genetic stability, and are easy to achieve high-throughput and automated analysis, but the detection cost is relatively high

Method used

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  • A method for assisted high-efficiency breeding of maize using ssr and snp molecular markers in combination
  • A method for assisted high-efficiency breeding of maize using ssr and snp molecular markers in combination
  • A method for assisted high-efficiency breeding of maize using ssr and snp molecular markers in combination

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0033] Example 1 The method of using SSR and SNP two kinds of molecular markers in combination to carry out maize assisted high-efficiency breeding

[0034] 1 Materials and methods

[0035] 1.1 Test materials

[0036] Maize inbred line Jing SD121 has high combining ability and excellent comprehensive traits, but has the disadvantages of high ear position and poor lodging resistance. The lodging resistance of JingSD121 was improved by backcrossing with JingSD127, an inbred line with low panicle position and good lodging resistance, which was closely related to JingSD121, as the donor parent and JingSD121 as the recurrent parent. In this example, 157 strains of BC initially selected through field phenotype selection were selected. 1 f 1 Generational backcross populations were used as research materials.

[0037] 1.2 DNA extraction

[0038] Seedlings of Jing SD127 and Jing SD121 were grown in indoor sand culture, and DNA was extracted at the 3-5 leaf stage. BC 1 f 1 On be...

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Abstract

The invention relates to a method of assistant efficient breeding of corns cooperatively using two molecular markers of SSR and SNP. The corn selfing line Jing SD127 is adopted as a donor parent while Jing SD121 is adopted as a recurrent parent; a BC1F1-generation group is obtained through backcross transformation; 6 SSR markers and 104 SNP markers are adopted for background selection for the BC1F1-generation group; and single plants with high background recovery rate are screened to increase the improvement speed of the corn selfing line Jing SD121. According to comparison of the background selection results of the SSR and SNP markers, the two markers are correlated in a middle degree. Therefore, the background selection can be performed by combining the SSR and SNP markers in the backcross early generation so as to realize large-group strict selection and improve the selection efficiency.

Description

technical field [0001] The invention relates to the fields of molecular biology and genetic breeding, in particular to a method for cooperating two molecular markers of SSR and SNP to carry out assisted high-efficiency breeding of maize. Background technique [0002] Conventional maize breeding selects target individuals through phenotypic observation, which not only relies on the experience of breeders, but also is often affected by the interaction between genotype and environment, making it difficult to select the ideal genotype. Molecular marker-assisted selection is an auxiliary means of applying molecular markers in the process of crop variety improvement, mainly including foreground selection for target traits and background selection for genetic background. Using molecular markers to assist background selection in the process of backcross breeding can significantly speed up the speed of background recovery and shorten the breeding time. [0003] Molecular markers sui...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895A01H1/04A01H1/02
CPCA01H1/02A01H1/04C12Q1/6895C12Q2600/156
Inventor 赵久然宋伟王凤格田红丽易红梅葛建镕李瑞媛王元东段民孝赵衍鑫张如养李春辉
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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