Specific primer and probe for real-time fluorescent PCR detection of enterococcus faecalis

Abstract

The invention provides a specific primer and probe for real-time fluorescent PCR detection of enterococcus faecalis. The specific primer and probe for real-time fluorescent PCR detection of the enterococcus faecalis are characterized in that the nucleotide sequences of the specific primer and probe is one of (1) or (2), for (1), an upstream primer is 5'-TTCGCACCAGGAGATAAA-3', a downstream primer is 5'-GCTGAATTGGCAGAGGAC-3', the probe is 5'-TTTTTCCCAGTTCACTGCCG-3', and the amplified target nucleotide sequence of the primer and probe are shown in SEQ ID No.3 in a sequence list; (2) is the sequence which is complementary to the sequence of (1). The specific primer and probe for real-time fluorescent PCR detection of the enterococcus faecalis can conduct qualitative and quantitative analysis on the clinically infected enterococcus faecalis content, has important significance on judging the clinically infected enterococcus faecalis content and plays an important role in the field of clinical tests.

Description

technical field [0001] The invention belongs to the technical field of microbial detection methods, and in particular relates to a set of specific primers and probes for real-time fluorescent PCR detection of Enterococcus faecalis. Background technique [0002] Enterococcus is a normal resident bacterium in human and animal intestines, and it is an important pathogenic bacterium of nosocomial infection. Among the infections caused by Gram-positive cocci, it ranks second. Enterococcus faecalis (Enterococcus faecalis) EF is a Gram-positive, catalase-negative coccus, which is the main flora in the intestinal tract of humans and animals. The bacterium can cause mass pathogenicity and hospital cross-infection, clinically manifested as urinary tract infection, bacteremia, endocarditis, etc. s concern. In human medicine, 85% to 90% of enterococcal infections are attributed to Enterococcus faecalis. Similarly, the impact of Enterococcus faecalis on animal breeding cannot be igno...

AI Technical Summary

Problems solved by technology

However, various studies have shown that phenotype-based methods often yield ambiguous results because of phenotypic and biochemical similarities between multiple Enterococcus species and that phenotypic methods take days to produce results

The results of detecting EF by immunoenzyme test, immunodiffusion method, latex agglutination test, immunofluorescence method and enzyme-linked immunosorbent assay are not satisfactory

Images