Application of snow chrysanthemum extract and snow chrysanthemum total flavone in preparation of drug for treating hepatic fibrosis

A liver fibrosis and extract technology, applied in the new application of snow chrysanthemum extract, in the field of preparation of medicines or functional foods for the treatment of liver fibrosis, can solve the problems of unretrieved snow chrysanthemum and snow chrysanthemum extract

Inactive Publication Date: 2016-06-08
TIANJIN UNIV OF COMMERCE
View PDF1 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there are no literature reports on the use of snow chrys

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of snow chrysanthemum extract and snow chrysanthemum total flavone in preparation of drug for treating hepatic fibrosis
  • Application of snow chrysanthemum extract and snow chrysanthemum total flavone in preparation of drug for treating hepatic fibrosis
  • Application of snow chrysanthemum extract and snow chrysanthemum total flavone in preparation of drug for treating hepatic fibrosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Cell Viability Test

[0042] Cells in the logarithmic growth phase were taken (the cell concentration was 7×10 3 / ml), inoculated in a 96-well plate with 100 μl per well, and cultured in an incubator at 37° C., saturated humidity, and 5% CO2. After the cells adhered to the wall, they were replaced with medium containing the total flavonoids of snow chrysanthemum with final concentrations of 0, 2.5, 5, 7.5, 10, 15, 20, and 30 mg / L to continue the culture. Set 6 replicate wells for each concentration and culture for 24 hours. Afterwards, 20 μl of MTT (5 mg / ml in PBS) was added to each well, and after culturing for 4 hours, 150 μl of DMSO was added to each well and shaken for 10 minutes. The absorbance value was measured at a wavelength of 460 nm on an enzyme-linked immunosorbent detector. According to the formula: cell growth inhibition rate = (blank group - snow chrysanthemum total flavonoids group / D blank group) × 100%, calculate the growth inhibition rate...

Embodiment 2

[0044] Example 2 Cell Apoptosis Experiment

[0045] 1×106 cells per well were inoculated in a 6-well plate. After the cells adhered to the wall, they were given different concentrations of total flavonoids of chrysanthemum, incubated for 24 hours, and LX- 2 Cells were stained with FITC-AnnexinV and PI, and the apoptosis rate was detected by flow cytometry. Cells were collected, put in an appropriate amount of lysate, lysed on ice for 30 min, centrifuged at 12000g at 4°C for 15 min, and the protein concentration was determined by BCA method. The samples were electrophoresed on 10% SDS-PAGE gel, transferred to PVDF membrane, blocked with 5% skimmed milk powder for 1 hour, coated with PARP antibody at a ratio of 1:1000, overnight at 4°C, and the corresponding secondary antibody was added after washing the membrane the next day, 37 Incubate at ℃ for 1 h, and expose to the chemiluminescence gel imaging system.

[0046] The result shows: if Figure 2A-1 , is a representative resu...

Embodiment 3

[0047] Example 3 The total flavonoids of snow chrysanthemum down-regulates the expression of fibrotic protein α-SMA and ECM (typeIcollagen) in LX-2 cells

[0048] Cells were collected, put in an appropriate amount of lysate, lysed on ice for 30 min, centrifuged at 12000g at 4°C for 15 min, and the protein concentration was determined by BCA method. Samples were electrophoresed on 10% SDS-PAGE gel, transferred to PVDF membrane, blocked with 5% skimmed milk powder for 1 hour, coated with primary antibody at a ratio of 1:1000, overnight at 4°C, and the corresponding secondary antibody was added after washing the membrane the next day, 37 Incubate at ℃ for 1 h, and expose to the chemiluminescent gel imaging system. The following antibodies are used as primary antibodies: anti-α-SMA, anti-ECM (typeIcollagen).

[0049] The results showed that the total flavonoids of Snow Chrysanthemum dose-dependently inhibited the expression of α-SMA and ECM-I collagen in hepatic stellate cells LX...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses application of snow chrysanthemum extract and snow chrysanthemum total flavone in preparation of a drug for treating hepatic fibrosis. According to a cell experiment and an animal experiment, the snow chrysanthemum extract is given to a hepatic stellate cell line LX-2 and a CC14-induced hepatic fibrosis rat model separately, and a cell experiment result shows that the snow chrysanthemum extract can significantly reduce the DNA binding capacity of NF-kappa B, lower fibrosis markers alpha smooth muscle actin and cell matrix protein I type collagen and promote LX-2 cell apoptosis; an in-vivo result shows that compared with the model group, the snow chrysanthemum extract can decrease the content of AST and ALT of a hepatic fibrosis rat, decrease the content of hepatic collagen and reduce expression of the alpha-SMA and an ECM, and after hepatic tissue is subjected to ultrasonication to form homogenate suspension, it can be found that the DNA binding capacity of the NF-kappa B is significantly reduced; the snow chrysanthemum extract can be used for preparing the drug or functional food for treating the hepatic fibrosis by serving as an NF-kappa B signal channel inhibitor.

Description

technical field [0001] The present invention relates to the new application of snow chrysanthemum, especially the new use of the snow chrysanthemum extract, that is, the new use in preparing medicine or functional food for treating liver fibrosis. Background technique [0002] Liver fibrosis is caused by chronic liver injury caused by various pathogenic factors, including chronic viral hepatitis, alcohol toxicity, autoimmune diseases, and genetic metabolic disorders, which cause abnormal proliferation of fibrous connective tissue in the liver and excessive precipitation of extracellular matrix. The main effector cells of liver fibrosis are hepatic stellate cells (HSCs). Under physiological conditions, HSCs are located in the space of Disse between the hepatic sinusoid and the wall of the hepatic sinusoid, and have functions such as storing vitamin A. However, HSCs are activated after liver injury, transform into myofibroblasts with contractile function, secrete smooth muscl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K36/28A61P1/16
CPCA61K36/28A61K2236/331A61K2236/39A61K2236/53
Inventor 赵辉只德贤张鹏
Owner TIANJIN UNIV OF COMMERCE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap