A fluorescent probe for detecting hydrogen peroxide and its preparation method and application

A hydrogen peroxide and fluorescent probe technology, which is applied in the field of fluorescent probes, can solve the problems that the selectivity of hydrogen peroxide needs to be improved, the selectivity of the probe is not good, and the in vivo imaging cannot be used, so as to achieve novel responsive groups, The synthesis method is simple and the effect of little interference
CN105647518BActive Publication Date: 2017-09-12SHANDONG NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANDONG NORMAL UNIV
Publication Date
2017-09-12

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Abstract

The invention discloses a fluorescent probe for detecting hydrogen peroxide and a preparation method and application thereof. The structural formula of the fluorescent probe for detecting hydrogen peroxide is shown in the description, wherein any one or two of R1-R5 represents electron withdrawing groups. The fluorescent probe is novel in group response, good in selectivity and high in sensitivity; the excitation emission wavelength of the fluorescent probe is located in the range of near infrared waves, damage to tissue is small, the penetrating power is high, and the fluorescent probe can be used for living body imaging. The preparation method comprises the steps that 3-aminophenol and Cy.7.Cl are subjected to a substitution reaction, and Cy-Ph-NH2 is obtained; a retro-Knoevenagel reaction is conducted, and P-NH2 is obtained; P-NH2 and a derivative of phenylglyoxylic acid are subjected to the substitution reaction, and the product P-R is obtained, that is, the fluorescent probe for detecting hydrogen peroxide is obtained. The synthesizing method is simple and suitable for industrial production.
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Description

technical field

[0001] The invention relates to a fluorescent probe, in particular to a new responsive group and a near-infrared dye fluorescent probe with good selectivity. The synthesis method and its application in detecting intracellular hydrogen peroxide belong to the technical field of detection. Background technique

[0002] Most of the hydrogen peroxide in the cell is produced by the mitochondrial respiratory chain, first oxygen (O 2 ) is reduced to generate O 2 · , then O 2 · It is converted into hydrogen peroxide by the dismutation of superoxide dismutases (SODs).

[0003] In living organisms, hydrogen peroxide is a major reactive oxygen species that plays an important role in cell signaling. Hydrogen peroxide can not only produce other active oxygen, but also is the product of other active oxygen oxidation reaction. Cells are stimulated to produce a burst of reactive oxygen species, which will affect several types of signaling proteins that can regulate cell...

Claims

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