Lung cancer screening kit
A technology of kits and reagents, applied in the field of lung cancer screening kits, to achieve good results in clinical application prospects
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[0021] Example 1 The relationship between the expression level of HSP60 and lung cancer
[0022] The experimental method is as follows:
[0023] 1. Clinical data
[0024] 15 patients with early stage lung cancer and 10 healthy controls were selected. The basic information is as follows:
[0025] Basic Information
[0026] 2. Detection of HSP60 expression level (quantitative detection of HSP60 content)
[0027] Using the AAH-BLG-CUST kit (catalog number: AAH-BLG-CUST) purchased from RayBiotech, the expression level of HSP60 was detected as follows:
[0028] 1. Sample dialysis
[0029] The blood of lung cancer patients and healthy controls was drawn, centrifuged (1200rpm, 10min) after anticoagulation with EDTA, the supernatant was aspirated and then centrifuged for the second time (3000rpm, 15min), and the supernatant after the second centrifugation, that is, plasma, was taken as the detection. Specimens were stored at -80°C after aliquoting.
[0030] Plasma samp...
Example Embodiment
[0059] Example 2 Composition of the kit of the present invention for detecting the expression level of HSP60 in blood and its use method
[0060] 1. Composition of HSP60 detection kit
[0061] Test kit (30 people):
[0062]
[0063] 2. How to use the HSP60 detection kit
[0064] The method of use is as follows:
[0065] 1. Sample dialysis
[0066] The blood of lung cancer patients and healthy controls was drawn, centrifuged (1200rpm, 10min) after anticoagulation with EDTA, the supernatant was aspirated and then centrifuged for the second time (3000rpm, 15min), and the supernatant after the second centrifugation, that is, plasma, was taken as the detection. Specimens were stored at -80°C after aliquoting.
[0067] Plasma samples were dialyzed using dialysis tubing prior to biotin labeling. 100 μL of samples were respectively taken and added to dialysis tubes, and then dialyzed in 4000 mL of 1×PBS (pH=8) at 4°C while stirring. The dialysate was changed every 3 hours, an...
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