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Schizothorax prenanti HSP60 (heat shock protein 60) gene cDNA (complementary deoxyribonucleic acid) full-length sequence and application thereof

A technology of Schizothorax and its application, applied in the direction of genetic engineering, plant gene improvement, application, etc., can solve the problems of Schizothorax HSP60 and Schizothorax not seen, and reach the prospect of clinical application Good, damage-reducing effect

Inactive Publication Date: 2016-12-07
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no report on the HSP60 gene of Schizothorax, and there is no report on the relationship between HSP60 and pathogen infection in Schizothorax

Method used

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  • Schizothorax prenanti HSP60 (heat shock protein 60) gene cDNA (complementary deoxyribonucleic acid) full-length sequence and application thereof
  • Schizothorax prenanti HSP60 (heat shock protein 60) gene cDNA (complementary deoxyribonucleic acid) full-length sequence and application thereof
  • Schizothorax prenanti HSP60 (heat shock protein 60) gene cDNA (complementary deoxyribonucleic acid) full-length sequence and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 cDNA Cloning of Schizothorax Hsp60 Gene

[0030] 1. Test materials

[0031] 1. Bacteria / carrier

[0032] Escherichia coli (Escherichia coli, E.coli) DH5α competent cells (purchased from Tiangen Biochemical Technology Co., Ltd., Beijing, China); pMD19-T (purchased from Bao Bioengineering Company, Dalian, China).

[0033] 2. Test animals

[0034] Healthy full-mouthed Schizothorax was purchased from Sichuan Runzhao Fishery Co., Ltd.

[0035] 3. Main reagents

[0036] (1) Sanprep Column Plasmid DNA Mini-Extraction Kit, Sanprep Column DNA Gel Recovery Kit, Sample RNA / DNA Protectant, RNAiso Plus Lysis Buffer, PrimeScript TM Reagent Kit (Perfect Real Time) cat#RR037A (purchased from Bao Biological Engineering Company, Dalian, China).

[0037] (2) 2×Taq Master Mix, Goldview, Agarose, DNA Marker (DL2000, DL1000 DNA Marker) (purchased from Tiangen Biochemical Technology Co., Ltd., Beijing, China).

[0038] (3)SMART TM RACE cDNA Amplification Kit (purchased from...

experiment example 1

[0144] Experimental Example 1 Changes in the expression level of Hsp60 gene after Streptococcus agalactiae infection

[0145] 1. Test materials

[0146] 1. Bacteria

[0147] Streptococcus agalactiae (Streptococcus agalactiae, S. agalactiae) was provided by the Fish Disease Research Center of Sichuan Agricultural University.

[0148] 2. Main reagents

[0149] Prime Script TM Reagent Kit With gDNA Eraser (Perfect Real Time) cat#RR047A, Premix Ex Taq TM II (Tli RNaseH Plus) (purchased from Bao Biological Engineering Co., Ltd., Dalian, China); Blood total protein, total RNA, DNA and microRNA-kit (purchased from Beijing Boling Kewei Biotechnology Co., Ltd.

[0150] 3. Main instruments

[0151] Step One Plus TM Real-time fluorescence quantitative PCR system-Life Tech (applied biosystems) (life-technologies company). Other instruments The instrument of Example 1.

[0152] 4. Test animals

[0153] Healthy full-mouthed Schizothorax was purchased from Sichuan Runzhao Fisher...

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Abstract

The invention provides a nucleotide sequence as shown in SEQ ID NO:1 and further provides application of the nucleotide sequence and a kit for detecting bacterial infection of schizothorax prenanti. The nucleotide sequence can serve as an auxiliary biomarker for detecting bacterial infection of schizothorax prenanti. By detection of Hsp60 mRNA expression level of blood, hepatopancreas, mesonephroi and or spleen tissues of schizothorax prenanti shoal, risk screening of bacterial infection of schizothorax prenanti can be realized, and the clinical application prospect is excellent.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a full-length cDNA sequence of the Schizothorax schizothorax HSP60 gene and an application thereof. Background technique [0002] Schizothorax prenanti (Schizothorax prenanti), commonly known as Ya fish, is one of the three wonders in Ya'an. It belongs to Cyprinidae, Cyprinidae, Schizothorax subfamily, and Schizothorax. It is distributed in the upper reaches of the Yangtze River, Dadu River, and Minjiang River. , Qingyi River, Jinsha River and the lower reaches of Wujiang River and other waters. Qikou Schizothorax has the characteristics of tender meat, delicious taste and rich nutritional value. It is a famous and high-quality aquaculture fish in my country and is favored by consumers. In the past 10 years, as a new aquaculture species, this fish has developed rapidly in Sichuan, Guizhou and Chongqing, and gradually formed an industry with local advantages. [0003] Ho...

Claims

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Application Information

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IPC IPC(8): C07K14/46C12N15/12C12Q1/68G01N33/68
CPCC07K14/461C12Q1/6888C12Q2600/158G01N33/6893
Inventor 陈德芳蒲云丹王虹彭爽李志琼汪开毓
Owner SICHUAN AGRI UNIV
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