A molecular detection method and special primers for the resistance of Chlorantraniliprole to Chlorantraniliprole
A technology for resistance and large borer, which is applied in the field of molecular detection and special primers for the resistance of giant borer to chlorantraniliprole, can solve problems such as affecting the safe production of rice, and achieve the effects of high sensitivity and delaying the development of resistance.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0016] Example 1: Application of special primers in the detection of resistant individuals of the overwintering generation of the large borer in Quzhou, Zhejiang Province
[0017] Primer design
[0018] According to the gene sequence (SEQ ID NO: 4) of the nicotine receptor gene of the large borer, self-designed allele-specific PCR primers are as follows:
[0019] Primer 1: 5'-TGTTGGATGTCGCTG G -3'; (SEQ ID NO: 1)
[0020] Primer 2: 5'-TGTTGGATGTCGCTG G -3'; (SEQ ID NO: 2)
[0021] Primer 3: 5'-GAAGAAGTTGAACGCGATCA-3' (SEQ ID NO: 3)
[0022] The primer design of the present invention does not rely on any primer design software, but is designed by itself according to the ryanodine receptor gene fragment to be amplified and the basic principles of primer design. Among them, primer 1 is an upstream primer dedicated to sensitivity, primer 2 is an upstream primer dedicated to resistance, and primer 3 is a downstream primer dedicated to sensitivity / resistance. The first...
Embodiment 2
[0026] Example 2: Application of special primers in the detection of resistance individuals of the overwintering generation of S. moerens in Jinhua, Zhejiang Province
[0027] Compared with Example 1, the primers and methods used are the same as those in Example 1 except that the region of origin of S. PCR amplification and sequencing of the ryanodine receptors of 30 overwintering individuals of S. chinensis collected from the Jinhua area of Zhejiang Province in 2016 (the method is the same as in Example 1), the results show that the homozygous individuals with the G4924E mutation, namely The number of individuals highly resistant to chlorantraniliprole was 0; the number of homozygous individuals without the G4924E mutation, that is, the number of individuals sensitive to chlorantraniliprole was 12, and the frequency was 40.0%; the heterozygous individuals were between chlorantraniliprole and The number of individuals between the formamide-sensitive type and the high-resis...
Embodiment 3
[0028] Example 3: Application of special primers in the detection of overwintering resistant individuals of S. arborescens in Wenling, Zhejiang Province
[0029] Compared with Example 1, the primers and methods used are the same as those in Example 1 except that the region of origin of S. The ryanodine receptors of 30 overwintering individuals of S. chinensis collected from Wenling area, Zhejiang Province in 2016 were amplified by PCR and verified by sequencing (the method was the same as that in Example 1). The results showed that the individuals homozygous for the G4924E mutation, namely The number of individuals highly resistant to chlorantraniliprole was 5, and the mutation frequency was 16.7%; the number of homozygous individuals without the G4924E mutation, that is, the number of individuals sensitive to chlorantraniliprole was 8, and the frequency was 26.7%; heterozygous individuals The number of individuals between the chlorantraniliprole-sensitive type and the high-...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com