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Method for detecting annual ring of desert plant haloxylon

A desert, annual ring technology, applied in the field of botany, can solve problems such as inability, slow growth, deciphering the history of climate change, etc.

Inactive Publication Date: 2016-07-06
NANTONG XIANGJIA TEXTILE SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the slow growth of Haloxylon Haloxylon, the phloem is very thin, there is no difference in the color of the trunk section, and no annual rings can be seen. It is difficult to calculate the age of the tree based on the annual rings, and it is impossible to decipher the history of climate change based on the annual rings.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Take the Haloxylon tree trunk in the arid part of the Gobi desert, cut it into 0.5 cm thick discs with a grinding wheel cutter, and polish the cut surface with a fine oil stone as a sample.

[0018] Weigh 50 mg of bismuth nitrate, add 20 ml of nitric acid with a mass percentage of 65% to dissolve the bismuth salt, then add 80 ml of 5% dilute sulfuric acid, and mix well. Measure 50ml, add 50ml of distilled water, mix evenly, and configure it as an alkaloid developer.

[0019] Use a fine brush made of polyvinyl chloride to evenly brush the alkaloid color developer on the sample, put it in a constant temperature box, and react at 35°C for 30 minutes, take out the sample and detect the annual rings under a biological microscope.

Embodiment 2

[0021] Take Haloxylon, a shrub in the driest part of the desert Gobi, cut a disc with a thickness of 0.5 cm from the trunk, and polish the disc with a fine oil stone to make a sample.

[0022] Accurately weigh 50 mg of bismuth nitrate and 20 ml of concentrated nitric acid, put them in an Erlenmeyer flask, shake gently to dissolve the bismuth nitrate, add 10 ml of glacial acetic acid, 70 ml of 5% dilute sulfuric acid, shake and mix well. Measure 50ml, add 50ml of distilled water, and mix evenly. It is a special color developer for Haloxylon alkaloids.

[0023] Use a fine brush to evenly paint the color developer on the sample, keep the temperature at 35°C for 30 minutes, and move it under a biological microscope to detect the annual rings of the tree. Use the digital camera on the biological microscope to take pictures of the annual rings on the sample, transfer them to the computer, and then study and interpret the history of climate change from the annual ring spacing.

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Abstract

The invention provides a method for detecting an annual ring of a desert plant haloxylon. According to the method, the annual ring of the haloxylon can be accurately measured, and the method specifically comprises the following detection steps: A, detecting a haloxylon standard sample, namely taking a haloxylon trunk growing in desert and gobi, cutting a flake sample, and polishing the sample to be smooth for later use; B, coating an alkaloid color developing agent on the sample, and acquiring the annual ring data after a clear annual ring appears on the sample surface. Alkali in the haloxylon is combined with the alkaloid color developing agent to present brownish red; the alkaloid color developing agent is an aqueous solution of bismuth salts and acids, wherein the content of bismuth element is 0.0001-0.04mol / L, and the concentration of hydrogen ion is 1.3-3.5mol / L. According to the detection method, chromatic aberration occurs in the trunk section, and the annual ring of the haloxylon can be clearly read out under a microscope.

Description

technical field [0001] The invention relates to a method for detecting annual rings of the desert plant Haloxylon, belonging to the field of botany. Background technique [0002] Haloxylon, a shrub growing in the Gobi desert. Detecting and determining the annual rings of Haloxylon is of great significance to deciphering the law of climate change and understanding the history of local climate change in decades and hundreds of years. Due to the extreme drought in the Gobi desert and the harsh conditions for plant growth, Haloxylon grows very slowly. A Haloxylon tree that has grown for decades or hundreds of years has a trunk as thick as a finger. The annual rings of ordinary tree trunks are clear and countable, and the local climate change history can be interpreted according to the number and width of the annual rings. Due to the slow growth of Haloxylon Haloxylon, the phloem is very thin, there is no difference in the color of the trunk section and no annual rings can be ...

Claims

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Application Information

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IPC IPC(8): G01N1/30G01N21/84
CPCG01N1/30G01N21/84G01N2001/302G01N2021/8466
Inventor 仲伟德
Owner NANTONG XIANGJIA TEXTILE SCI & TECH CO LTD
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