Preservation method of Dendrobium candidum seeds

A kind of Dendrobium officinale, preservation method technology

Inactive Publication Date: 2016-07-13
SUBTROPICAL CROPS INST OF GUIZHOU PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a kind of preservation method of dendrobium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Follow these steps:

[0009] 1. Selection of centrifuge tubes: Select high-quality polypropylene screw centrifuge tubes with a specification of 5ml.

[0010] 2. Sterilize the centrifuge tube: put the centrifuge tube through a high pressure sterilizer at 121 o C high temperature sterilization for 30 minutes.

[0011] 3. Select the capsules of Dendrobium officinale: The capsules of Dendrobium officinale should be normal in color, plump, mature, free from diseases and insect pests, free from damage, and not cracked.

[0012] 4. Sterilized seed packaging: Soak the collected Dendrobium officinale capsules with 75% alcohol on the ultra-clean workbench for 30 seconds, rinse them with sterile water for 3 times, and then sterilize them with 0.1% mercuric chloride solution for 10 minutes. Rinse 5 times with bacterial water, soak the capsules in 75% alcohol for 3 minutes, hold the capsules with tweezers, burn the alcohol on the surface of the capsules on an alcohol lamp, and pla...

Embodiment 2

[0015] Embodiment 2: carry out according to the following steps:

[0016] 1. Selection of centrifuge tubes: Select high-quality polypropylene screw centrifuge tubes with a specification of 5ml.

[0017] 2. Sterilize the centrifuge tube: put the centrifuge tube through a high pressure sterilizer at 125 o C high temperature sterilization for 25 minutes.

[0018] 3. Select the fruit of Dendrobium candidum: The capsule of Dendrobium candidum should be normal in color, plump, mature, free from diseases and insect pests, without damage, and without cracking.

[0019] 4. Sterilized seed packaging: Soak the collected Dendrobium officinale capsules in 75% alcohol for 30 seconds on the ultra-clean workbench, rinse with sterile water twice, soak the capsules in 75% alcohol for 5 minutes, and use tweezers Place the alcohol on the dry surface of the capsules on an alcohol lamp and place them in a sterilized small stainless steel dish. Gently cut the top of the capsule with a sterilized ...

Embodiment 3

[0022] Embodiment 3: carry out according to the following steps:

[0023] 1. Selection of centrifuge tubes: Select high-quality polypropylene screw centrifuge tubes with a specification of 5ml.

[0024] 2. Sterilize the centrifuge tube: put the centrifuge tube through a high pressure sterilizer at 125 o C high temperature sterilization for 20 minutes.

[0025] 3. Select the fruit of Dendrobium candidum: The capsule of Dendrobium candidum should be normal in color, plump, mature, free from diseases and insect pests, without damage, and without cracking.

[0026] 4. Seed sterilization sub-packaging: Soak the collected Dendrobium officinale capsules with 75% alcohol for 30 seconds on the ultra-clean workbench, rinse them with sterile water for 5 times, and then sterilize them with 0.1% mercuric chloride solution for 10 minutes. Rinse 4 times with sterile water, dry the surface moisture of the capsules with sterile filter paper, and place them in a sterilized sterile stainless s...

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PUM

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Abstract

The invention discloses a preservation method of Dendrobium candidum seeds.The preservation method includes: collecting Dendrobium candidum capsules which are full, mature and non-cracked; performing surface sterilization on the capsules; on a super clean bench, using a sterile operation method to cut open the top ends of the capsules, pouring seeds into a high-quality polypropylene screwed centrifugal tube sterilized by a high-pressure sterilization pot, and sealing for preservation.By using the preservation method to preserve the Dendrobium candidum seeds, the objective of long-time preservation can be achieved, and the seeds do not go bad and still have vitality.The germination rate reaches higher than 90% after sowing the seeds preserved by the method, so that the preservation method is worthy of popularization.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a method for preserving dendrobium officinale seeds with a centrifuge tube. Background technique [0002] The fruit of Dendrobium officinale is a capsule, which contains tens of thousands of powdery seeds. Artificial breeding of Dendrobium candidum seedlings is obtained by aseptic sowing of seeds, so it is particularly important to obtain well-preserved and mature Dendrobium candidum seeds. However, in actual production, if the collected whole capsules are directly stored in a refrigerator at 4°C, the epidermis of the capsules is easily softened and cracked, which is not conducive to the operation of aseptic sowing, resulting in a short storage time, which generally cannot exceed one month. moon. Therefore, existing Dendrobium candidum seed preservation technology still has deficiency. Contents of the invention [0003] The purpose of the present invention is to provid...

Claims

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Application Information

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IPC IPC(8): A01C1/00
CPCA01C1/00
Inventor 罗晓青王晓敏
Owner SUBTROPICAL CROPS INST OF GUIZHOU PROVINCE
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