Unlock instant, AI-driven research and patent intelligence for your innovation.

A lectin chip and kit for identifying breast cancer based on salivary protein and its application

A salivary protein and lectin technology is applied in the field of lectin chips and kits for identifying breast cancer based on proteins, which can solve the problems of early screening of women with breast cancer that have not yet been used, and achieves simple and easy collection and avoids health problems. effect of risk

Active Publication Date: 2017-12-01
深圳格道糖生物技术有限公司
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above studies all used tissue or serum as samples, and little is known about the changes in protein glycosylation levels in saliva, and it has not been applied to the early screening of breast cancer women

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A lectin chip and kit for identifying breast cancer based on salivary protein and its application
  • A lectin chip and kit for identifying breast cancer based on salivary protein and its application
  • A lectin chip and kit for identifying breast cancer based on salivary protein and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] The present invention will be further described by the specific examples given below, but not as a limitation of the present invention.

[0025] The present invention screens lectin probes for screening female patients with breast cancer from 37 lectins (as shown in Table 1).

[0026] Table 1

[0027]

[0028]

[0029] The reagent materials used in the present invention include: protease inhibitors, DMSO, Cy3, Cy5 fluorescent dyes, Sephadexg G-25 column, bovine serum albumin (BSA), glass substrate, oxysilane reagent (GPTS), Bradford reagent, PVDF membrane, Tween-20, and common reagents of domestic analytical grade.

[0030] The equipment used includes: ultra-fast refrigerated centrifuge 5804R: Eppendorf, Germany; micronucleic acid and protein analyzer: Implen, Germany; chip spotting instrument: Boao Jingxin SmartArrayer48 spotting instrument; biochip scanner 4000B: Axon, USA; chip Hybridization Box HL-2000: American UVP Company. Immobilon-P semi-dry film transf...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A lectin chip and kit for identifying breast cancer based on salivary proteins and its application, relates to a lectin chip for screening cancer, specifically relates to a lectin chip and kit for identifying breast cancer based on protein and its application . The purpose is to provide a non-damaging lectin chip and kit for identifying breast cancer through changes in sugar chains in saliva and applications thereof. The lectin chip for identifying breast cancer based on salivary proteins includes a test lectin probe set, which at least includes PHA-E+L, LTL, BS-I, MAL-I, LCA, BPL, A combination of PTL‑II, NPA, GSL‑I or at least a combination including LTL, BS‑I, MAL‑I, LCA, BPL, NPA, GSL‑I. The lectin chip can quickly detect the differentially expressed glycoprotein sugar chain structures in patient saliva samples, determine whether the corresponding group of people suffers from breast tumors, and determine whether it is breast cancer; in addition, saliva is used as the detection substance in the present invention , the collection is simple and easy, and avoids the health risks existing in the existing detection through serum collection samples.

Description

technical field [0001] The invention relates to a lectin chip for discriminating cancer, in particular to a lectin chip for discriminating breast cancer based on protein and a kit thereof. Background technique [0002] Breast cancer is the most common type of malignant tumor in the world, which seriously endangers the physical and mental health of women. In recent years, the incidence of breast cancer in my country has shown a clear upward trend. IARC predicts that the number of female breast cancer patients in my country will reach 234,000 in 2030, and the mortality rate will increase by 47.94% compared with 2008. Due to the rising incidence rate and young age of onset Advances in breast cancer screening, diagnosis, and treatment have been receiving much attention due to the globalization and diversification of the disease. In areas with high breast cancer incidence in Europe and the United States, the reduction in mortality has benefited to a certain extent from the census...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/68
CPCG01N33/57415G01N33/68
Inventor 李铮刘夏薇于汉杰钟耀刚舒健阳佳君
Owner 深圳格道糖生物技术有限公司