Method for improving germination percentage of jatamans valeriana rhizome
A spider incense and germination rate technology, which is applied in the fields of seed and rhizome treatment, application, agriculture, etc., can solve the problems of difficult collection of spider incense seeds and low germination rate, and achieves the effect of low cost, simple operation and solving difficult collection.
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Embodiment 1
[0032] 1. Seed collection
[0033] ① time
[0034] From late March to early May,
[0035] ② Record the date of listing on the flowering plants every day,
[0036] ③ 7 days after flowering, use 30-35 mesh transparent gauze to sew a 35 cm x 35 cm bag, and put it on the inflorescence.
[0037] ④ When the seed coat of the seeds is yellowish brown, take back the bagging before the flower stems wither and fall, and ripen the seeds for 3-5 days. After the inflorescences are dry in the shade, shake the seeds out.
[0038] ⑤ Use an 8-mesh sieve to screen out larger impurities, and then use an 18-mesh sieve to get new species of spider incense for later use.
Embodiment 2
[0040] Seed constant temperature treatment
[0041] ① Routinely disinfect the new species of spider incense, soak in distilled water at 30°C for 6-12 hours,
[0042] ②Put it in a sterilized Petri dish (9 cm in diameter) with filter paper, add appropriate amount of distilled water to keep the filter paper and seeds moist, and seal it with a film.
[0043] ③ Place the sterilized culture dish in a constant temperature incubator for cultivation. The temperature set in the constant temperature incubator is 0°C-30°C, preferably 5°C-30°C, 20°C-25°C, 25°C. Each treatment was repeated 3 times, 100 grains per dish. Observe and record the germination of the seeds.
[0044] The processing results are shown in Table 1
[0045] temperature (°C) Germination rate(%) 0 0 5 4.9 10 13.4 15 17.2 20 19.8 25 20.5 30 12.6
[0046] It can be seen from Table 1 that under the constant temperature condition, the germination of the seeds of the spide...
Embodiment 3
[0048] Seed variable temperature treatment
[0049] Set up 12 variable temperature treatments, each treatment has 3 repetitions, and 100 grains per dish. Observe and record the germination of the seeds.
[0050] ① Routinely disinfect the new species of spider incense, put them in the freezer at -5°C, -10°C or -15°C for 7 days, 14 days, 21 days and 28 days respectively,
[0051] ② Take out the seeds and soak them in distilled water at 30°C for 6-12 hours.
[0052] ③ Place in a sterilized Petri dish (9 cm in diameter) with filter paper, add appropriate amount of distilled water to keep the filter paper and seeds moist, and seal with a film.
[0053] ④ Place the sterilized culture dish in a constant temperature incubator at 25°C for cultivation.
[0054] The processing results are shown in Table 2
[0055] variable temperature treatment Germination rate(%) -15°C treatment for 7 days +25°C 45.4 -15℃ for 14 days +25℃ 50.7 -15°C treatment for 21 days...
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