Preparation method for algae spot samples, algae spot samples and method for enhancing microalgae P700 signals

A technology of algae spots and samples, which is applied in the field of biological instrument testing, can solve problems affecting the application value of P700 related measuring instruments, flaws in microalgae P700 measurement data, and inability to obtain P700 signals, etc. Wide, avoid the effect of violent fluctuations

Inactive Publication Date: 2016-09-28
上海泽泉科技股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Measuring only the P700 signal at the end of microalgal growth cannot reflect the photosynthetic properties of the often more important early growth of microalgae
This makes microalgae P700 measurement data seriously flawed
[0007] 2. It takes days or even weeks for microalgae to grow from a thin pre-growth stage to a dense end-growth stage, which takes too long
[0008] 3. Even at the end of the growth stage of microalgae with high algae cell density, its chlorophyll concentration is still much lower than that of leaves and other high chlorophyll samples, so the P700 signal is still weak
In addition, the movement of algae cells in the suspension will also cause great interference to the P700 signal, and the ideal P700 signal cannot be obtained.
[0009] The P700 measurement technology of microalgae samples has become a bottleneck limiting the research of microalgae photosynthesis, and has seriously affected the application value of P700 related measurement instruments in the field of algae photosynthesis. A simple and effective method for enhancing the P700 signal of microalgae is urgently needed

Method used

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  • Preparation method for algae spot samples, algae spot samples and method for enhancing microalgae P700 signals
  • Preparation method for algae spot samples, algae spot samples and method for enhancing microalgae P700 signals
  • Preparation method for algae spot samples, algae spot samples and method for enhancing microalgae P700 signals

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preparation example Construction

[0054] The present invention provides an embodiment of a preparation method of algae spot sample, refer to figure 1 shown; includes:

[0055] In step S10, the microalgae suspension is dripped onto the filter membrane to reach a preset concentration;

[0056] In step S20, in step S10, algae spots are formed after being filtered by a filter membrane, and samples of algae spots whose diameter is not smaller than the diameter of the probe are obtained.

[0057] Specifically, since the difference between the oxidized state and the reduced state of P700 on the 700nm light absorption is very small, the P700 measuring instrument needs to have high sensitivity. Chlorophyll content is low, it is difficult to detect. Therefore, it is easier to obtain a good P700 signal for materials with higher chlorophyll content, such as plant leaves. However, for thin microalgae suspensions with high transparency and low absorbance, the P700 signal will be very weak. In addition, the algae cells ar...

Embodiment 1

[0082] The dilute microalgae suspension samples in the early growth stage were measured by a Dual-PAM-100 P700 measuring instrument. refer to Figure 4 As shown; the P700 signal of the thin microalgae suspension sample is due to the low concentration of algae cells, so the signal noise is huge, and valuable information cannot be obtained.

Embodiment 2

[0084] The dense microalgae suspension samples at the end of growth stage were measured by Dual-PAM-100 P700 measuring instrument. refer to Figure 5 As shown; the P700 signal of the dense microalgae suspension sample is due to the increase of the concentration of algae cells in the sample, so the noise of P700 is reduced, and the resolution of the signal is improved to a certain extent. However, due to the low concentration of algae cells and the movement of algae cells in the suspension, the signal still has large fluctuations, which cannot meet high-precision analysis and application.

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Abstract

The invention discloses a preparation method of an algal spot sample, a method for the algal spot sample and a method for enhancing the P700 signal of a microalgae, wherein the preparation method of the algal spot sample comprises: step S10, dripping a microalgae suspension on a filter membrane to achieve a pre- set the concentration; in step S20, in step S10, algal plaques are formed after being filtered by a filter membrane, and algal plaque samples with a diameter not less than the diameter of the probe are obtained; in step S30, the algal plaque samples are placed in a colorless transparent bag; in step S40, the In step S30, the colorless transparent bag provided with the algal plaque sample is sealed after the air is discharged. The microalgae suspension can be made into algal plaque samples by the invention, the signal intensity of P700 can be greatly improved, the signal noise can be reduced, and the invention has the characteristics of simple operation, low cost and time saving.

Description

technical field [0001] The invention relates to the technical field of biological instrument testing, in particular to a method for preparing algae spot samples, an algae spot sample and a method for enhancing microalgae P700 signals. Background technique [0002] Photosynthesis is the most important chemical reaction on earth, and its process is extremely complex. The measurement and research of photosynthesis has always been a research hotspot in the fields of plant physiology, plant ecology, agronomy, forestry, horticulture, and algal physiology and ecology. [0003] Photosynthesis can be roughly divided into two parts: light reaction and dark reaction. Among them, the photoreaction occurs on the thylakoid membrane, which is composed of multiple photoreaction-related components, mainly photosystem I and photosystem II. P700 is the photosynthetic reaction center of photosystem I, which is composed of chlorophyll and binding protein. It is named for its absorption peak at...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/28
Inventor 郭峰顾群
Owner 上海泽泉科技股份有限公司
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