Check patentability & draft patents in minutes with Patsnap Eureka AI!

A kind of tissue culture method of duck celery

A technology of tissue culture and culture medium, applied in the field of agricultural cultivation, can solve the problems of not being suitable for mass reproduction, low seed germination rate, long time, etc., and achieve the effects of improving induction, good rooting effect, and increasing yield

Active Publication Date: 2017-11-21
JIAXING VOCATIONAL TECHN COLLEGE
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the introduction and cultivation method of this celery takes a long time, and the germination rate of the seeds is low, so it is not suitable for mass propagation.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of tissue culture method of duck celery
  • A kind of tissue culture method of duck celery
  • A kind of tissue culture method of duck celery

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A kind of tissue culture method of celery of the present embodiment comprises the following steps:

[0028] (1) Disinfection treatment of explants: Select well-grown annual tender stems of celery, soak them in washing powder for 10 minutes, rinse them in tap water for 3 hours, and sterilize them with 75% ethanol for 20-40 seconds on an ultra-clean workbench. Rinse 3 to 5 times, then sterilize with NaClO with a mass concentration of 1.5 to 3.5% for 5 to 10 minutes, rinse with sterile water for 3 to 5 times, dry the surface water with sterilized filter paper, and finally put the sterilized duck Celery is placed in a sterile and clean petri dish for later use. Disinfection of explants can kill various microorganisms on the surface of the material. At the same time, the above-mentioned disinfection treatment has strong disinfection ability, is not easy to remain, and is harmless to the environment.

[0029] (2) Callus induction: Cut the sterilized tender stems of celery in ...

Embodiment 2

[0037] A kind of tissue culture method of celery of the present embodiment comprises the following steps:

[0038](1) Disinfection treatment of explants: Select well-grown annual tender stems of celery, soak them in washing powder for 10 minutes, rinse them in tap water for 3 hours, and sterilize them with 75% ethanol for 20-40 seconds on an ultra-clean workbench. Rinse 3 to 5 times, then sterilize with NaClO with a mass concentration of 1.5 to 3.5% for 5 to 10 minutes, rinse with sterile water for 3 to 5 times, dry the surface water with sterilized filter paper, and finally put the sterilized duck Celery is placed in a sterile and clean petri dish for later use. Disinfection of explants can kill various microorganisms on the surface of the material. At the same time, the above-mentioned disinfection treatment has strong disinfection ability, is not easy to remain, and is harmless to the environment.

[0039] (2) Callus induction: Cut the sterilized tender stems of celery in s...

Embodiment 3

[0047] A kind of tissue culture method of celery of the present embodiment comprises the following steps:

[0048] (1) Disinfection treatment of explants: Select well-grown annual tender stems of celery, soak them in washing powder for 10 minutes, rinse them in tap water for 3 hours, and sterilize them with 75% ethanol for 20-40 seconds on an ultra-clean workbench. Rinse 3 to 5 times, then sterilize with NaClO with a mass concentration of 1.5 to 3.5% for 5 to 10 minutes, rinse with sterile water for 3 to 5 times, dry the surface water with sterilized filter paper, and finally put the sterilized duck Celery is placed in a sterile and clean petri dish for later use. Disinfection of explants can kill various microorganisms on the surface of the material. At the same time, the above-mentioned disinfection treatment has strong disinfection ability, is not easy to remain, and is harmless to the environment.

[0049] (2) Callus induction: Cut the sterilized tender stems of celery in ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for tissue culture of celery. The tissue culture of celery in the prior art has the problems of low lateral bud induction rate, callus cannot continue to differentiate to produce adventitious buds, and mass propagation of celery cannot be carried out. A tissue culture method of celery, comprising the following steps: (1) explant disinfection treatment; (2) callus induction; (3) differentiation culture; (4) rooting culture; (5) seedling hardening and transplantation plant. The method of the invention is used for the tissue culture of celery, overcomes the disadvantages of long dormant period and low germination rate, and has the advantages of fast reproduction speed, large reproduction coefficient, uniform offspring and high transplant survival rate. It can meet the requirements of large-scale production, and can effectively maintain the excellent genetic traits of the female parent, ensuring the quality and quality of seedlings.

Description

technical field [0001] The invention relates to a method for breeding celery, in particular to a tissue culture method for celery, belonging to the technical field of agricultural cultivation. Background technique [0002] Cryptotaenia japonica Hassk.f.japonica belongs to Umbelliferae, belongs to the genus Cryptotaenia, and is a perennial herb. It usually grows in mountains, ravines and forest areas at an altitude of 200-2400 meters. It is distributed in all provinces (districts) in the north and south of my country, as well as in Korea and Japan. [0003] The whole herb can be used as medicine to treat weakness, anuria and swollen poison, etc. The folks use the whole herb to mash and apply externally to treat snake bites. The seeds contain about 22% oil and can be used to make soap and paint. Duck celery is one of the important cultivated vegetables in Japan. It mainly eats tender stems and leaves, which have a special flavor, and are mainly used as soup stock or made i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 许陶昀陈超何杭凤陈丹萍张平
Owner JIAXING VOCATIONAL TECHN COLLEGE
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com